文献类型： 外文期刊

作者： Singh, Rajesh Kumar ¹ ; Singh, Pratiksha ³ ; Sharma, Anjney ¹ ; Guo, Dao-Jun ¹ ; Upadhyay, Sudhir K. ⁵ ; Song, Qi-Qi ⁶ ; Verma, Krishan K. ¹ ; Li, Dong-Ping ⁷ ; Malviya, Mukesh Kumar ¹ ; Song, Xiu-Peng ² ; Yang, Li-Tao ² ; Li, Yang-Rui ¹ ;

作者机构： 1.Guangxi Acad Agr Sci, Chinese Acad Agr Sci, Sugarcane Res Ctr,Minist Agr,Guangxi Key Lab Suga, Sugarcane Res Inst,Key Lab Sugarcane Biotechnol &, Nanning 530007, Peoples R China

2.Guangxi Key Lab Crop Genet Improvement & Biotechn, Nanning 530007, Peoples R China

3.Guangxi Univ Nationalities, Sch Marine Sci & Biotechnol, Nanning 530008, Peoples R China

4.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China

5.VBS Purvanchal Univ, Dept Environm Sci, Jaunpur 222003, India

6.Guangxi Subtrop Crop Res Inst, Sugarcane Res Inst, Nanning 530001, Peoples R China

7.Guangxi Acad Agr Sci, Microbiol Inst, Nanning 530007, Peoples R China

关键词： endophytic diazotrophs; high-throughput sequencing; microbial diversity; nitrogen fixation; nifH gene; sugarcane

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 11 期

页码：

收录情况： SCI

摘要： Sugarcane (Saccharum officinarum L.) is one of the world's highly significant commercial crops. The amounts of synthetic nitrogen (N-2) fertilizer required to grow the sugarcane plant at its initial growth stages are higher, which increases the production costs and adverse environmental consequences globally. To combat this issue, sustainable environmental and economic concerns among researchers are necessary. The endophytic diazotrophs can offer significant amounts of nitrogen to crops through the biological nitrogen fixation mediated nif gene. The nifH gene is the most extensively utilized molecular marker in nature for studying N-2 fixing microbiomes. The present research intended to determine the existence of novel endophytic diazotrophs through culturable and unculturable bacterial communities (EDBCs). The EDBCs of different tissues (root, stem, and leaf) of five sugarcane cultivars (Saccharum officinarum L. cv. Badila, S. barberi Jesw.cv Pansahi, S. robustum, S. spontaneum, and S. sinense Roxb.cv Uba) were isolated and molecularly characterized to evaluate N-2 fixation ability. The diversity of EDBCs was observed based on nifH gene Illumina MiSeq sequencing and a culturable approach. In this study, 319766 operational taxonomic units (OTUs) were identified from 15 samples. The minimum number of OTUs was recorded in leaf tissues of S. robustum and maximum reads in root tissues of S. spontaneum. These data were assessed to ascertain the structure, diversity, abundance, and relationship between the microbial community. A total of 40 bacterial families with 58 genera were detected in different sugarcane species. Bacterial communities exhibited substantially different alpha and beta diversity. In total, 16 out of 20 genera showed potent N-2-fixation in sugarcane and other crops. According to principal component analysis (PCA) and hierarchical clustering (Bray-Curtis dis) evaluation of OTUs, bacterial microbiomes associated with root tissues differed significantly from stem and leaf tissues of sugarcane. Significant differences often were observed in EDBCs among the sugarcane tissues. We tracked and validated the plethora of individual phylum strains and assessed their nitrogenase activity with a culture-dependent technique. The current work illustrated the significant and novel results of many uncharted endophytic microbial communities in different tissues of sugarcane species, which provides an experimental system to evaluate the biological nitrogen fixation (BNF) mechanism in sugarcane. The novel endophytic microbial communities with N-2-fixation ability play a remarkable and promising role in sustainable agriculture production.