您好，欢迎访问广西农业科学院 机构知识库！

The carboxypeptidase B and carbonic anhydrase genes play a reproductive regulatory role during multiple matings in Ophraella communa

文献类型： 外文期刊

作者： Chen, Guangmei 1 ; Gao, Xuyuan 2 ; Zhang, Yan 1 ; Ma, Chao 1 ; Ma, Weihua 4 ; Zhou, Zhongshi 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China

2.Chinese Acad Agr Sci, Natl Nanfan Res Inst, Sanya, Peoples R China

3.Guangxi Acad Agr Sci, Inst Plant Protect, Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning, Peoples R China

4.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China

关键词： multiple mating; seminal fluid protein genes; female reproductive tract; reproduction; Ophraella communa

期刊名称：FRONTIERS IN MOLECULAR BIOSCIENCES （ 影响因子：5.0； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 10 卷

页码：

收录情况： SCI

摘要： Seminal fluid proteins (SFPs) are key factors in sexual reproduction and are transferred to females during mating with sperm. SFPs have a nutritional value because they protect and activate sperm storage and release to optimize fecundity. Multiple matings promote ovipositioning in several insect species. Therefore, insects may obtain more SFP through multiple matings to maximize reproduction, but this process has not yet been clearly confirmed. Here, the relationship between multiple matings and the SFPs in Ophraella communa (Coleoptera: Chrysomelidae), a biological control agent of the common ragweed Ambrosia artemisiifolia (Asterales: Asteraceae), was studied. Multiple matings significantly increased female fecundity and ovary egg deposition. Carboxypeptidase B (OcCpb) and carbonic anhydrase (OcCa) genes were identified as putative SFP genes in O. communa and they showed strong male-biased expression. Additionally, OcCpb and OcCa expression was upregulated in the bursa copulatrix of mating females compared to that in virgin females, but their expression gradually declined after copulation. Furthermore, OcCpb and OcCa knockdown in males led to a decrease in insect fecundity compared to that in the control. The reproductive tract of females mated with dsRNA-treated males was dissected and observed and, notably, the ovaries produced significantly fewer eggs. These data suggest that OcCpb and OcCa play regulatory roles during multiple matings in O. communa.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台