Improved growth and quality of Siraitia grosvenorii plantlets using a temporary immersion system
文献类型： 外文期刊
作者： Yan, Huabing 1 ; Liang, Chunxiu 2 ; Li, Yangrui 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Biotechnol Res Inst, Nanning 530007, Guangxi, Peoples R China
2.Guangxi Univ, Coll Agr, Nanning 530007, Guangxi, Peoples R China
关键词： Siraitia grosvenorii fruit derivatives: antineoplastic-drug;antiviral-drug;antiinfective-drug;callus formation;quality improvement;shoot length;dry weight;proliferation rate;rhizogenesis;fresh weight;growth improvement;total biomass production;large-scale commercial production;well-developed root system;natural-like plantl;et;adequate culture ventilation
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）
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收录情况： SCI
摘要： The effects of temporary immersion system (TIS) culture on the growth and quality of Siraitia grosvenorii plantlets were investigated. The TIS promoted the growth and quality of S. grosvenorii plantlets. Proliferation rate, shoot length, fresh weight (FW) and dry weight (DW) of shoots, and total biomass production were significantly (P a parts per thousand currency sign 0.05) higher in the TIS than in gelled and liquid medium, respectively. The TIS also decreased callus formation at the base of shoots. Callus diameter was significantly (P a parts per thousand currency sign 0.05) lower in the TIS (3.30 mm) than in gelled medium (6.31 mm) and liquid medium (6.77 mm), respectively. FW (50.83 mg) and DW (7.08 mg) of callus in the TIS were also significantly (P a parts per thousand currency sign 0.05) lower than those in gelled medium (80.00 and 10.56 mg, respectively) and liquid medium (218.75 and 23.75 mg, respectively). During rhizogenesis, minimal callus was evident at the base of shoots in the TIS, with a well-developed root system. However, the plantlets in gelled medium just produced thick, brown and easily broken roots with obvious callus and fewer secondary roots. The natural-like plantlets of S. grosvenorii obtained in the TIS would probably have positive effects on ex vitro rooting and transplanting in large-scale commercial production.
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