您好，欢迎访问广西农业科学院 机构知识库！

Comparative Transcriptome Analysis of Pueraria lobata Provides Candidate Genes Involved in Puerarin Biosynthesis and Its Regulation

文献类型： 外文期刊

作者： Xi, Huiting 1 ; Zhu, Yaru 1 ; Sun, Wenwen 1 ; Tang, Nan 1 ; Xu, Zhengqin 1 ; Shang, Xiaohong 3 ; Zhang, Yansheng 1 ; Yan, Huabing 3 ; Li, Changfu 1 ;

作者机构： 1.Shanghai Univ, Res Ctr Nat Prod, Plant Sci Ctr, Sch Life Sci, Shanghai 200444, Peoples R China

2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty Ag, Wuhan Bot Garden, Wuhan 430074, Peoples R China

3.Guangxi Acad Agr Sci, Cash Crops Res Inst, Nanning 530007, Peoples R China

关键词： Pueraria lobata (wild); Pueraria lobata var; thomsonii; puerarin; C-glucosyltransferase; MYB transcription factor

期刊名称：BIOMOLECULES （ 影响因子：5.5； 五年影响因子：5.8 ）

ISSN：

年卷期： 2023 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Pueraria lobata is a traditional Chinese herb in which an isoflavone C-glucoside, namely puerarin, has received the utmost interest due to its medicinal properties. To date, the biogenesis of puerarin, especially its C-glucosyl reaction in the pathway, remains poorly understood. Moreover, the transcription factors (TFs) that regulate puerarin biosynthesis in P. lobata have not been reported. Here, we performed phytochemical studies on the different developmental stages of the root, stem, and leaf tissues of two P. lobata cultivars, which suggested that both the roots and stems of P. lobata were the sites of puerarin biosynthesis. RNA-sequencing was conducted with the root and stem tissues of the two cultivars under different stages, and the clean reads were mapped to the recently published genome of P. lobata var. thomsonii, yielding the transcriptome dataset. A detailed analysis of the gene expression data, gene coexpression network, and phylogeny proposed several C-GTs that likely participate in puerarin biosynthesis. The first genome-wide analysis of the whole MYB superfamily in P. lobata presented here identified a total of 123 nonredundant PlMYB genes that were significantly expressed in the analyzed tissues. The phylogenetic analysis of PlMYBs with other plant MYB proteins revealed strong PlMYB candidates that may regulate the biosynthesis of isoflavones, such as puerarin.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台