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MaNAC029 modulates ethylene biosynthesis and fruit quality and undergoes MaXB3-mediated proteasomal degradation during banana ripening

文献类型： 外文期刊

作者： Wei, Wei 1 ; Yang, Ying-ying 1 ; Chen, Jian-ye 1 ; Lakshmanan, Prakash 2 ; Kuang, Jian-fei 1 ; Lu, Wang-jin 1 ; Shan, Wei 1 ;

作者机构： 1.South China Agr Univ, Engn Res Ctr Southern Hort Prod Preservat, State Key Lab Conservat & Utilizat Subtrop Agrobio, Minist Educ,Coll Hort,Guangdong Prov Key Lab Posth, Guangzhou 510642, Peoples R China

2.Guangxi Acad Agr Sci, Guangxi Key Lab Sugarcane Genet Improvement, Minist Agr, Sugarcane Res Inst,Key Lab Sugarcane Biotechnol &, Nanning 530007, Peoples R China

3.Southwest Univ, Interdisciplinary Res Ctr Agr Green Dev Yangtze Ri, Chongqing 400716, Peoples R China

4.Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld 4067, Australia

关键词： Banana fruit; Ripening; Ethylene; Fruit quality formation; NAC; E3 ligase

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：10.7； 五年影响因子：11.4 ）

ISSN： 2090-1232

年卷期： 2023 年 53 卷

页码：

收录情况： SCI

摘要： Introductions: Ethylene regulates ripening by activating various metabolic pathways that controlcolor, aroma, flavor, texture, and consequently, the quality of fruits. However, the modulation of ethylene biosynthesis and quality formation during banana fruit ripening remains unclear.Objectives: The present study aimed to identify the regulatory module that regulates ethylene and fruit quality-related metabolisms during banana fruit ripening. Methods: We used RNA-seq to compare unripe , ripe banana fruits and identified a ripening-induced NAC transcription factor, MaNAC029. We further performed DNA affinity purification sequencing to iden-tify the MaNAC0290s target genes involved in ethylene biosynthesis and fruit quality formation , elec-trophoretic mobility shift assay, chromatin immunoprecipitation with real-time polymerase chain reaction and dual luciferase assays to explore the underlying regulatory mechanisms. Immunoprecipitation combined with mass spectrometry, yeast two-hybrid assay, and bimolecular fluo-rescence complementation assay were used to screen and verify the proteins interacting with MaNAC029. Finally, the function of MaNAC029 and its interacting protein associated with ethylene biosynthesis and quality formation was verified through transient overexpression experiments in banana fruits.Results: The study identified a nucleus-localized, ripening-induced NAC transcription factor MaNAC029. It transcriptionally activated genes associated with ethylene biosynthesis and a variety of cellular meta-bolisms related to fruit quality formation (cell wall degradation, starch degradation, aroma compound synthesis, and chlorophyll catabolism) by directly modulating their promoter activity during ripening. Overexpression of MaNAC029 in banana fruits activated ethylene biosynthesis and accelerated fruit ripen-ing and quality formation. Notably, the E3 ligase MaXB3 interacted with and ubiquitinated MaNAC029 protein, facilitating MaNAC029 proteasomal degradation. Consistent with this finding, MaXB3 overex-pression attenuated MaNAC029-enhanced ethylene biosynthesis and quality formation.Conclusion: Our findings demonstrate that a MaXB3-MaNAC029 module regulates ethylene biosynthesis and a series of cellular metabolisms related to fruit quality formation during banana ripening. These results expand the understanding of the transcriptional and post-translational mechanisms of fruit ripening and quality formation.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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