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Identification of passion fruit HSF gene family and the functional analysis of PeHSF-C1a in response to heat and osmotic stress

文献类型： 外文期刊

作者： Wang, Lulu 1 ; Liu, Yanhui 2 ; Chai, Gaifeng 4 ; Zhang, Dan 4 ; Fang, Yunying 4 ; Deng, Kao 4 ; Aslam, Mohammad 4 ; Niu, Xiaoping 4 ; Zhang, Wenbin 5 ; Qin, Yuan 3 ; Wang, Xiaomei 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Hort Res Inst, Nanning Invest Stn South Subtrop Fruit Trees, Minist Agr, Nanning 530007, Peoples R China

2.Longyan Univ, Coll Life Sci, Longyan 364000, Peoples R China

3.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangxi Key Lab Sugarcane Biol, Nanning 530004, Guangxi, Peoples R China

4.Fujian Agr & Forestry Univ, Pingtan Inst Sci & Technol, Coll Agr, Coll Life Sci, Fuzhou 350002, Fujian, Peoples R China

5.Fine Variety Breeding Farm Xinluo Dist, Longyan 364000, Peoples R China

关键词： HSF genes; Transcription factor; Gene expression; Abiotic stress; Passiflora edulis

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 200 卷

页码：

收录情况： SCI

摘要： Heat stress transcription factors (HSFs) are the major regulators of plant response to environmental stress, especially heat and drought stress. To gain a deeper understanding of the mechanisms underlying HSFs in the abiotic stress response of passion fruit, we conducted an in silico analysis of the HSF gene family. Through bioinformatics and phylogenetic analyses, we identified 18 PeHSF members and classified them into A, B, and C groups. Collinearity analysis results revealed that the expansion of the PeHSF gene family was due to the presence of segmental duplication. Furthermore, gene structure and protein domain analysis illustrated that PeHSFs in the same subgroup are relatively conserved. Conserved motif and function domain analysis suggested that PeHSF proteins possess typical conserved functional domains of the HSF family. A protein interaction network and 3D structure prediction were used to study the potential regulatory relationship of PeHSFs. Additionally, the sub -cellular localization results of PeHSF-A6a, PeHSF-B4b, and PeHSF-C1a were consistent with the predictions. RNA-seq and RT-qPCR analysis revealed the expression patterns of PeHSFs in different tissues of passion fruit floral organs. Promoter analysis and the expression patterns of the PeHSFs under different treatments demonstrated their involvement in various abiotic stress processes. Notably, overexpression of PeHSF-C1a consistently enhanced tolerance to drought and heat stress in Arabidopsis. Overall, our findings provide a scientific basis for further functional studies of PeHSFs that could contribute to improvement of passion fruit breeding.

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