Epigenetic modification mechanisms of chloroplasts mutants in pineapple leaves during somatic regeneration
文献类型： 外文期刊
作者： Liu, Yanhui 1 ; Priyadarshani, S. V. G. N. 1 ; Chi, Meirong 1 ; Yan, Maokai 3 ; Mohammadi, Mohammad Aqa 1 ; Zhang, Man 1 ; Zhou, Qiao 1 ; Wang, Lulu 3 ; Luo, Tiantian 1 ; Wai, Myat Hnin 1 ; Wang, Xiaomei 1 ; Cai, Hanyang 1 ; Wang, Haifeng 3 ; Qin, Yuan 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Ctr Genom & Biotechnol, Key Lab of Genet,Breeding & Multiple Utilizat Crop, Fuzhou 350002, Fujian, Peoples R China
2.Longyan Univ, Coll Life Sci, Longyan 364012, Fujian, Peoples R China
3.Guangxi Univ, Coll Agr, Guangxi Key Lab Sugarcane Biol, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530004, Guangxi, Peoples R China
4.Guangxi Acad Agr Sci, Minist Agr, Hort Res Inst, Nanning Invest Stn South Subtrop Fruit Trees, Nanning 530007, Guangxi, Peoples R China
5.Fujian Agr & Forestry Univ, Pingtan Sci & Technol Res Inst, Fuzhou 350002, Fujian, Peoples R China
6.Natl Educ Commiss, Colombo, Sri Lanka
关键词： Pineapple; DNA methylation; Transcriptome; Chlorophyll metabolism
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：5.4 ）
ISSN： 2095-9885
年卷期： 2023 年 9 卷 3 期
页码：
收录情况： SCI
摘要： Somaclonal variation in tissue culture is a common phenomenon induced by various external or internal environmental conditions, resulting in heritable or non-heritable alterations in gene expression. One crucial mechanism involved in plant growth and development is epigenetic regulation. A highly dynamic epigenome can respond to environmental changes by regulating gene expression. DNA methylation is one of these epigenetic modifications that can alter gene expression in tissue-cultured pineapple plants. The underlying mechanism of such somaclonal variations in pineapple and the epigenetic regulation involvement in somaclonal variations has not been studied. This study performed DNA methylome and transcriptome sequencing of wild-type (WT) and mutant pineapple plants (WS, HW, and TW). We observed altered DNA methylation patterns in chlorophyll development in the mutants. Specifically, we noticed that the methylation levels in the CHG and CHH contexts were lower in the gene body regions compared to the upstream and downstream regions. We identified several thousand differentially methylated regions (DMRs) located at the gene body regions, some of which overlapped with the differentially expressed genes (DEGs). Functional enrichment analyses suggested that these genes were involved in chlorophyll metabolism. Thus, our results revealed that the transcriptional regulation of many chlorophyll metabolic essential genes could be regulated by DNA methylation caused by somaclonal variations and provided insights into epigenetic mechanisms underlying the regulation of chlorosis in pineapple plants.
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