Citrus exosome-modified exogenous dsRNA delivery reduces plant pathogen resistance and mycotoxin production
文献类型： 外文期刊
作者： Yin, Chunxiao 1 ; Lao, Yuli 1 ; Xie, Lihong 3 ; Chen, Lianfei 4 ; Jiang, Yueming 1 ; Gong, Liang 1 ;
作者机构： 1.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China
2.Gannan Normal Univ, Coll Life Sci, Ganzhou 341000, Jiangxi, Peoples R China
3.Guangxi Acad Agr Sci, Agrofood Sci & Technol Res Inst, Nanning 530007, Peoples R China
4.Guangdong Agr Technol Extens Ctr, Guangzhou 510515, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
关键词： Fungicide resistance; RNAi; Exosomes; dsRNA delivery; Mycotoxin
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）
ISSN： 0048-3575
年卷期： 2024 年 205 卷
页码：
收录情况： SCI
摘要： Plant-derived exosome-like nanoparticles (PENs) are crucial for intercellular communication. However, PENbased transport of pathogenic fungal genes remains unclear. This study isolated and purified PENs from lane late navel orange citrus juice by following the sucrose gradient ultracentrifugation technique. Citrus PENs were round and oval-shaped with an average size of 154.5 +/- 1.9 nm. Electroporation-based exogenous dsRNA to PENs loading efficiency remained at 6.0 %. Laser confocal microscopy was employed to investigate citrus PEN uptake by fungal spores. dsCrcB loaded PENs inhibited the CrcB gene expression in spores to alleviate Penicillium italicum resistance against prochloraz fungicide, which promoted resistant strains' mortality by 10-fold. Moreover, dsFUM21-loaded PENs suppressed the FUM21 gene expression in spores, which significantly reduced FB1 production in Fusarium proliferatum. These findings suggest that citrus PENs could potentially serve as nano-carriers to counter fungicide resistance and mycotoxin production in pathogenic plant fungi.
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