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Downregulated expression of S-2-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo

文献类型： 外文期刊

作者： Hu, Jianbing 1 ; Xu, Qiang 1 ; Liu, Chenchen 1 ; Liu, Binghao 2 ; Deng, Chongling 2 ; Chen, Chuanwu 2 ; Wei, Zhuangmin 3 ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China

2.Guangxi Acad Specialty Crops, Guangxi Engn Res Ctr Citrus Breeding & Culture, Guilin 541004, Peoples R China

3.Guangxi Subtrop Crops Res Inst, Nanning 530001, Peoples R China

4.Guangxi Acad Agr Sci, Hort Res Inst, Minist Agr, Nanning Invest & Expt Stn South Subtrop Fruit Tre, Nanning 530007, Peoples R China

5.Hunan Acad Agr Sci, Hort Inst, Changsha 410125, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：6.793； 五年影响因子：6.589 ）

ISSN： 2662-6810

年卷期： 2021 年 8 卷 1 期

页码：

收录情况： SCI

摘要： Self-incompatibility (SI) substantially restricts the yield and quality of citrus. Therefore, breeding and analyzing self-compatible germplasm is of great theoretical and practical significance for citrus. Here, we focus on the mechanism of a self-compatibility mutation in 'Guiyou No. 1' pummelo (Citrus maxima), which is a spontaneous mutant of 'Shatian' pummelo (Citrus maxima, self-incompatibility). The rate of fruit set and the growth of pollen tubes in the pistil confirmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of 'Guiyou No. 1'. Segregation ratios of the S genotype in F-1 progeny, expression analysis, and western blotting validated that the reduced levels of S-2-RNase mRNA contribute to the loss of SI in 'Guiyou No. 1'. Furthermore, we report a phased assembly of the 'Guiyou No. 1' pummelo genome and obtained two complete and well-annotated S haplotypes. Coupled with an analysis of SV variations, methylation levels, and gene expression, we identified a candidate gene (CgHB40), that may influence the regulation of the S-2-RNase promoter. Our data provide evidence that a mutation that affects the pistil led to the loss of SI in 'Guiyou No. 1' by influencing a poorly understood mechanism that affects transcriptional regulation. This work significantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes.

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