文献类型： 外文期刊
作者： Liu, Chenchen 1 ; Zheng, Xin 1 ; Hu, Jianbing 1 ; Xu, Qiang 1 ; Wen, Hao 1 ; Zhang, Zhezhong 1 ; Liu, Ran 1 ; Chen, Xiangling 3 ; Xie, Zongzhou 1 ; Ye, Junli 1 ; Deng, Xiuxin 1 ; Chai, Lijun 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan 430070, Peoples R China
2.Hubei Hongshan Lab, Wuhan 430070, Peoples R China
3.Guangxi Acad Agr Sci, Hort Res Inst, Nanning Invest & Expt Stn South Subtrop Fruit Tree, Minist Agr, Nanning 530007, Peoples R China
关键词： Self-incompatibility; Citrus; RNA-Seq; S-RNase; CgHSFB1
期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：3.9； 五年影响因子：4.6 ）
ISSN： 0167-4412
年卷期： 2024 年 114 卷 4 期
页码：
收录情况： SCI
摘要： As self-incompatibility is a major issue in pummelo breeding and production, its mechanism in citrus was analyzed to improve breeding efficiency and reduce production costs. Rutaceae belongs to S-RNase type of gametophytic self-incompatibility. While the function of S-RNase/SLF and the mechanism of self-incompatibility have been studied extensively, the transcriptional regulation of S-RNase has been less studied. We performed transcriptome sequencing with the styles of 'Shatian' pummelo on the day of anthesis and 1-5 days before anthesis, and found that the transcript level of S-RNase gradually decreased with flower development. By analyzing differentially expressed genes and correlation with the expression trend of S-RNase, we identified a candidate gene, CgHSFB1, and utilized biochemical experiments such as yeast one-hybrid assay, electrophoretic mobility shift assay and dual-luciferase assay, as well as transient transformation of citrus calli and Citrus microcarpa and demonstrated that CgHSFB1 could directly bind to the S-1-RNase promoter and repress the expression of S-1-RNase, which is involved in the pummelo self-incompatibility response. In contrast, CgHSFB1 did not bind to the promoter of S-2-RNase, and there was specificity in the regulation of S-RNase. Key message Transcription factor CgHSFB1 regulates the expression of S -RNase and is thus involved in self-incompatibility in citrus.
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