文献类型： 外文期刊

作者： Hu, Jianbing ¹ ; Liu, Chenchen ¹ ; Du, Zezhen ¹ ; Guo, Furong ¹ ; Song, Dan ¹ ; Wang, Nan ¹ ; Wei, Zhuangmin ³ ; Jiang, Jingdong ¹ ; Cao, Zonghong ¹ ; Shi, Chunmei ¹ ; Zhang, Siqi ¹ ; Zhu, Chenqiao ¹ ; Chen, Peng ⁴ ; Larkin, Robert ¹ ; Lin, Zongcheng ¹ ; Xu, Qiang ¹ ; Ye, Junli ¹ ; Deng, Xiuxin ¹ ; Bosch, Maurice ⁵ ; Franklin-Tong, Vernonica ⁶ ; Chai, Lijun ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, Wuhan, Peoples R China

2.Hubei Hongshan Lab, Wuhan, Peoples R China

3.Guangxi Subtrop Crops Res Inst, Nanning, Peoples R China

4.Hunan Acad Agr Sci, Hort Inst, Changsha, Peoples R China

5.Aberystwyth Univ, Inst Biol Environm & Rural Sci IBERS, Aberystwyth, Wales

6.Univ Birmingham, Coll Life & Environm Sci, Sch Biosci, Birmingham, England

关键词： Self-incompatibility; citrus; S-RNase; S-locus; MITE; evolution

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Self-incompatibility (SI) is a widespread prezygotic mechanism for flowering plants to avoid inbreeding depression and promote genetic diversity. Citrus has an S-RNase-based SI system, which was frequently lost during evolution. We previously identified a single nucleotide mutation in S-m-RNase, which is responsible for the loss of SI in mandarin and its hybrids. However, little is known about other mechanisms responsible for conversion of SI to self-compatibility (SC) and we identify a completely different mechanism widely utilized by citrus. Here, we found a 786-bp miniature inverted-repeat transposable element (MITE) insertion in the promoter region of the FhiS(2)-RNase in Fortunella hindsii Swingle (a model plant for citrus gene function), which does not contain the S-m-RNase allele but are still SC. We demonstrate that this MITE plays a pivotal role in the loss of SI in citrus, providing evidence that this MITE insertion prevents expression of the S-RNase; moreover, transgenic experiments show that deletion of this 786-bp MITE insertion recovers the expression of FhiS(2)-RNase and restores SI. This study identifies the first evidence for a role for MITEs at the S-locus affecting the SI phenotype. A family-wide survey of the S-locus revealed that MITE insertions occur frequently adjacent to S-RNase alleles in different citrus genera, but only certain MITEs appear to be responsible for the loss of SI. Our study provides evidence that insertion of MITEs into a promoter region can alter a breeding strategy and suggests that this phenomenon may be broadly responsible for SC in species with the S-RNase system.