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Development of transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay for visual detection of citrus tatter leaf virus

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文献类型：外文期刊

作者： Zeng, Ting ¹ ; Chen, XiangLing ² ; Liao, Ping ¹ ; Gao, HaiXing ¹ ; Zheng, CaiRong ¹ ; HuangFu, MengYang ¹ ; Zhou, Yan ¹ ;

作者机构： 1.Southwest Univ, Citrus Res Inst, Natl Citrus Engn & Technol Res Ctr, Chongqing 400712, Peoples R China

2.Guangxi Acad Agr Sci, Hort Res Inst, Nanning 530007, Peoples R China

关键词： Citrus tatter leaf virus (CTLV); Recombinase polymerase amplification-lateral-flow dipstick test strip; Rapid visual detection

期刊名称：JOURNAL OF VIROLOGICAL METHODS （影响因子：2.623；五年影响因子：2.239 ）

ISSN： 0166-0934

年卷期： 2022 年 309 卷

页码：

收录情况： SCI

摘要： The citrus tatter leaf virus (CTLV) is one of the most destructive citrus viral diseases worldwide. In this study, reverse transcription-recombinase polymerase amplification combined with a lateral flow dipstick (RT-RPA-LFD) assay for rapid visual detection of CTLV was established. The assay was performed at 35 ? in 27 min without specialised equipment. The RT-RPA-LFD assay showed high specificity to CTLV, and the sensitivity to CTLV was the same as that of quantitative RT-PCR at 3 x 10(3) copies/mu L CTLV RNA transcripts. A total of 45 field tangor samples were tested using RT-RPA-LFD, RT-PCR, and RT-qPCR, and the results were consistent. The results demonstrated that the RT-RPA-LFD assay is a promising tool for rapid on-site CTLV detection.

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