文献类型： 外文期刊

作者： Fatima, Samia ¹ ; Khan, Muhammad Omar ¹ ; Iqbal, Nadia ¹ ; Iqbal, Muhammad Mudassar ¹ ; Qamar, Huma ² ; Imtiaz, Muhammad ¹ ; Hundleby, Penny ⁴ ; Wei, Zhengyi ⁵ ; Ahmad, Niaz ¹ ;

作者机构： 1.Pakistan Inst Engn & Appl Sci PIEAS, Natl Inst Biotechnol & Genet Engn Coll NIBGE C, Faisalabad 38000, Pakistan

2.Ayub Agr Res Inst, Oilseeds Res Inst, Faisalabad 38000, Pakistan

3.Univ Western Australia, Sch Biol Sci, Crawley, WA 6009, Australia

4.Norwich Res Pk, John Innes Ctr, Dept Crop Genet, Norwich NR4 7UH, England

5.Guangxi Acad Agr Sci, Maize Res Inst, Nanning 530007, Peoples R China

关键词： glucosinolate transporters; glucosinolates; gene expression; Brassica; salt tolerance; antioxidants

期刊名称：METABOLITES （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Brassica crops are well known for the accumulation of glucosinolates-secondary metabolites crucial for plants' adaptation to various stresses. Glucosinolates also functioning as defence compounds pose challenges to food quality due to their goitrogenic properties. Their disruption leaves plants susceptible to insect pests and diseases. Hence, a targeted reduction in seed glucosinolate content is of paramount importance to increase food acceptance. GLUCOSINOLATE TRANSPORTERS (GTRs) present a promising avenue for selectively reducing glucosinolate concentrations in seeds while preserving biosynthesis elsewhere. In this study, 54 putative GTR protein sequences found in Brassica were retrieved, employing Arabidopsis GTR1 and GTR2 templates. Comprehensive bioinformatics analyses, encompassing gene structure organization, domain analysis, motif assessments, promoter analysis, and cis-regulatory elements, affirmed the existence of transporter domains and stress-related regulatory elements. Phylogenetic analysis revealed patterns of conservation and divergence across species. Glucosinolates have been shown to increase under stress conditions, indicating a potential role in stress response. To elucidate the role of GTRs in glucosinolate transportation under NaCl stress in two distinct Brassica species, B. juncea and B. napus, plants were subjected to 0, 100, or 200 mM NaCl. Based on the literature, key GTR genes were chosen and their expression across various plant parts was assessed. Both species displayed divergent trends in their biochemical profiles as well as glucosinolate contents under elevated salt stress conditions. Statistical modelling identified significant contributors to glucosinolate variations, guiding the development of targeted breeding strategies for low-glucosinolate varieties. Notably, GTR2A2 exhibited pronounced expressions in stems, contributing approximately 52% to glucosinolate content variance, while GTR2B1/C2 displayed significant expression in flowers. Additionally, GTR2A1 and GTR1A2/B1 demonstrated noteworthy expression in roots. This study enhances our understanding of glucosinolate regulation under stress conditions, offering avenues to improve Brassica crop quality and resilience.