文献类型： 外文期刊

作者： Rizwan, Muhammad ² ; Mostofa, Mohammad Golam ³ ; Ahmad, Muhammad Zulfiqar ⁴ ; Zhou, Yaoyu ⁵ ; Adeel, Muhammad ⁶ ; M ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Resources & Environm, Microelement Res Ctr, Wuhan 430070, Hubei, Peoples R China

2.Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434023, Peoples R China

3.Bangabandhu Sheikh Mujibur Rahman Agr Univ, Dept Biochem & Mol Biol, Gazipur 1706, Bangladesh

4.Gomal Univ, Fac Agr, Dept Plant Breeding & Genet, Dera Ismail Khan, KP, Pakistan

5.Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China

6.China Agr Univ, Coll Resources & Environm Sci, Beijing Key Lab Farmland Soil Pollut Prevent & Re, Beijing 100193, Peoples R China

7.Guangzhou Univ, Sch Civil Engn, Guangzhou 51006, Guangdong, Peoples R China

8.Shenyang Univ, Minist Educ, Key Lab Ecorestorat Reg Contaminated Environm, Shenyang 11044, Liaoning, Peoples R China

9.Natl Univ Med Sci, Dept Multidisciplinary Studies, Rawalpindi 46000, Pakistan

10.Huazhong Agr Univ, Coll Plant Sci & Technol, Stat Genom Lab, Wuhan 430070, Hubei, Peoples R China

11.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China

关键词： Chloroplast; Genes expressions; Hydrogen sulfide; Nickel toxicity; Nitrogen metabolism; Photosynthesis; Rice

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2019 年 138 卷

页码：

收录情况： SCI

摘要： Hydrogen sulfide (H2S) modulates plant tolerance to abiotic stresses, but its regulatory effects on nitrogen metabolism and chloroplast protection under nickel (Ni) stress in crop plants remain elusive. Taking this into account, we investigated the potential roles of sodium hydrosulfide (NaHS), a H2S generator, in the improvement of growth performance of rice plants under Ni stress. Results showed that NaHS successfully reversed the adverse effects of Ni, as reflected in plant growth and biomass, and photosynthesis attributes including photosynthetic rates, stomatal conductance, transpiration rate, internal CO2 concentration and photosynthetic pigment contents. NaHS generated H2S plays a crucial role in controlling the photosynthetic machinery of rice as evidenced by the ultrastructure of chloroplast viewed under transmission electron microscope (TEM). The reduced content of Ni in roots and leaves of NaHS-supplemented Ni-stressed plants has revealed the restricted uptake and accumulation of Ni. A rescue of NaHS to the Ni-induced decline in nitrate (NO3-) content and the activities NO3- biosynthesizing enzymes nitrate reductase, nitrite reductase, glutamate synthase, glutamate oxaloacetate transaminase, glutamine synthetase, and glutamate pyruvate transaminase in leaves indicated a positive role of H2S on NO3- metabolism in rice under Ni stress. NaHS application also reverted Ni-mediated increases in ammonium (NH4+) content and glutamate dehydrogenase activity, implying H2S-induced alleviation of NH4+ toxicity. The regulatory effects of H2S on nitrogen metabolism was further confirmed by increased and decreased transcript abundance of NO3- and NH4+ metabolism associated genes, respectively. Our study suggests a decisive role of H2S in controlling Ni toxicity as elucidated by the novel findings such as enhanced gas exchanged parameters, Ni homeostasis and chloroplast protection. Moreover, this article highlights the significance of H2S in controlling chloroplast biogenesis and nitrogen metabolism in rice crop under Ni stress.