Construction of a High-Density Genetic Map for Pitaya Using the Whole Genome Resequencing Approach
文献类型： 外文期刊
作者： Wu, Zhijiang 1 ; Deng, Haiyan 1 ; Liang, Guidong 1 ; Ye, Xiaoying 1 ; Qin, Yonghua 2 ; Huang, Lifang 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Hort Res Inst, Nanning 530007, Peoples R China
2.South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
3.Minist Agr, Key Lab South China Hort Crop Biol & Germplasm En, Guangdong Prov Key Lab Postharvest Sci Fruits & V, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China
关键词： genetic map; pitaya; genome resources; crop improvement; marker assisted breeding
期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）
ISSN：
年卷期： 2021 年 7 卷 12 期
页码：
收录情况： SCI
摘要： Pitaya (Hylocereus undatus) is one of the most economic fleshy fruit tree crops. This study aimed at producing a high-density linkage genetic map of pitaya based on the whole genome resequencing (WGrS) approach. For this purpose, a bi-parental F1 population of 198 individuals was generated and genotyped by WGrS. High-quality polymorphic 6434 single polymorphism nucleotide (SNP) markers were extracted and used to construct a high-density linkage map. A total of 11 linkage groups were resolved as expected in accordance with the chromosome number. The map length was 14,128.7 cM with an average SNP interval of 2.2 cM. Homology with the sequenced reference genome was described, and the physical and genetic maps were compared with collinearity analysis. This linkage map in addition to the available genomic resources will help for quantitative trait mapping, evolutionary studies and marker-assisted selection in the important Hylocereus species.
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