A transcriptional repressor BrDof2.4 regulates protease genes involved in postharvest leaf senescence in Chinese flowering cabbage
文献类型： 外文期刊
作者： Wang, Ci-mei 1 ; Zeng, Ze-xiang 1 ; Su, Xin-guo 2 ; Lakshmanan, Prakash 3 ; Shan, Wei 1 ; Kuang, Jian-fei 1 ; Lu, Wang 1 ;
作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Postharvest Sci Fruits & V, State Key Lab Conservat & Utilizat Subtrop AgroBi, Coll Hort,Engn Res Ctr Southern Hort Prod Preserv, Guangzhou 510642, Peoples R China
2.Guangdong AIB Polytech, Guangzhou 510507, Peoples R China
3.Guangxi Acad Agr Sci, Sugarcane Res Inst, Key Lab Sugarcane Biotechnol & Genet Improvement, Minist Agr, Nanning 530007, Peoples R China
4.Guangxi Acad Agr Sci, Sugarcane Res Inst, Guangxi Key Lab Sugarcane Genet Improvement, Nanning 530007, Peoples R China
5.Southwest Univ, Coll Resources & Environm, Interdisciplinary Res Ctr Agr Green Dev Yangtze R, Chongqing 400716, Peoples R China
6.Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld 4067, Australia
关键词： Chinese flowering cabbage; Protease; Dof; Transcriptional repressor; Leaf senescence
期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：5.537； 五年影响因子：5.821 ）
ISSN： 0925-5214
年卷期： 2021 年 181 卷
页码：
收录情况： SCI
摘要： Protein degradation is an integral process of leaf senescence, and this catabolism is strongly associated with proteases in diverse plant species. However, the characterization of proteases as well as their upstream regulators during postharvest senescence, especially in economically important leafy vegetables such as Chinese flowering cabbage, remains to be determined. In this study, temporal and spatial gene expression patterns of eight proteases showed that three of them, namely, BrAPM1, BrASPG2 and BrSAG12, were most up-regulated during postharvest leaf senescence. Their expressions were also significantly induced by senescence-accelerating phytohormones abscisic acid (ABA) and methyl jasmonate (MeJA), but were inhibited by senescence-delaying hormones gibberellin (GA3) and cytokinin (6-BA). Importantly, using yeast one-hybrid screening, we identified a Dof (DNA-binding with one finger) transcription factor, BrDof2.4, as the putative binding protein of BrAPM1, BrASPG2 and BrSAG12 promoters. Nucleus-localized BrDof2.4 was senescence-, ABA- and MeJArepressible, but was GA3- and 6-BA-inducible. Furthermore, gel mobility shift and transient dual luciferase reporter assays revealed that BrDof2.4 suppressed the transcription of BrAPM1, BrASPG2 and BrSAG12, through binding to their promoters. Taken together, our findings demonstrate that BrDof2.4 acts as a potential repressor of postharvest leaf senescence in Chinese flowering cabbage by the direct suppression of protease expression. Our work contributes towards improving the technology for increasing the shelf-life and commercial value of this important leafy vegetable.
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