HprK(Xcc) is a serine kinase that regulates virulence in the Gram-negative phytopathogen Xanthomonas campestris
文献类型： 外文期刊
作者： Li, Rui-Fang 1 ; Cui, Ping 1 ; Wei, Ping-Zhen 1 ; Liu, Xing-Yan 1 ; Tang, Ji-Liang 1 ; Lu, Guang-Tao 1 ;
作者机构： 1.Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Guangxi, Peoples R China
2.Guangxi Acad Agr Sci, Plant Protect Res Inst, Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning, Guangxi, Peoples R China
期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.491； 五年影响因子：6.438 ）
ISSN： 1462-2912
年卷期：
页码：
收录情况： SCI
摘要： The HprK serine kinase is a component of the phosphoenolpyruvate phosphotransferase system (PTS) of bacteria that generally regulates catabolite repression through phosphorylation/dephosphorylation of the PTS protein PtsH at a conserved serine residue. However, many bacteria do not encode a complete PTS or even have an HprK homologue. Xanthomonas campestris pv. campestris (Xcc) is a pathogen that cause black rot disease in crucifer plants and one of the few Gram-negative bacteria that encodes a homologue of HprK protein (herein HprK(Xcc)). To gain insight into the role of HprK(Xcc) and other PTS-related components in Xcc we individually mutated and phenotypically assessed the resulting strains. Deletion of hprK(Xcc) demonstrated its requirement for virulence and other diverse cellular processes associated including extracellular enzyme activity, extracellular-polysaccharide production and cell motility. Global transcriptome analyses revealed the HprK(Xcc) had a broad regulatory role in Xcc. Additionally, through overexpression, double gene deletion and transcriptome analysis we demonstrated that hprK(Xcc) shares an epistatic relationship with ptsH. Furthermore, we demonstrate that HprK(Xcc) is a functional serine kinase, which has the ability to phosphorylate PtsH. Taken together, the data illustrates the previously unappreciated global regulatory role of HprK(Xcc) and previously uncharacterized PTS components that control virulence in this pathogen.
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