您好，欢迎访问广西农业科学院 机构知识库！

XrvB regulates the type III secretion system by directly repressing hrpG transcription in Xanthomonas oryzae pv. oryzicola

文献类型： 外文期刊

作者： Peng, Jian-Ling 1 ; Shi, Jia-Feng 1 ; Ma, Zeng-Feng 2 ; Zhou, Xiao-Long 2 ; Ye, Wen-Xin 1 ; Su, Qian 1 ; Zhu, Gui-Ning 3 ; Tang, Ji-Liang 1 ; Li, Rui-Fang 3 ; Lu, Guang-Tao 1 ;

作者机构： 1.Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530004, Peoples R China

2.Guangxi Acad Agr Sci, Rice Res Inst, Nanning 530007, Peoples R China

3.Guangxi Acad Agr Sci, Plant Protect Res Inst, Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning 530007, Peoples R China

关键词： Xanthomonas oryzae; T3SS; hrp genes; H-NS protein; Xrv protein

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.4； 五年影响因子：4.2 ）

ISSN： 2096-5362

年卷期： 2023 年 5 卷 1 期

页码：

收录情况： SCI

摘要： Xrv proteins are a group of regulators in Xanthomonas spp., belonging to the histone-like nucleoid-structuring (H-NS) proteins of Gram-negative bacteria. The rice bacterial leaf streak pathogen Xanthomonas oryzae pv. oryzicola (Xoc) harbors three Xrv proteins, the XrvA, XrvB, and XrvC. Here, we report that in Xoc, the XrvB but not XrvA and XrvC is involved in negative regulation of the type III secretion system (T3SS) encoded by hrp genes. As with other Xanthomonas spp., the T3SS is an essential virulence determinant of Xoc and the expression of the hrp genes in Xoc is controlled by the HrpG/HrpX regulatory cascade. HrpG positively regulates the expression of HrpX, which in turn activates the transcription of the hrp genes. We provide evidences to demonstrate that the XrvB binds to the promoter region of hrpG and represses its transcription. Furthermore, we found that XrvB production was induced in the Xoc cells cultured in a nutrient-rich medium compared to a hrp-inducing minimal medium. We also found that in Xoc, the hrpG expression level is inversely correlated with the content of XrvB, and XrvB occupancy at hrpG promoter region is positively correlated with XrvB levels. Our data suggest that XrvB is a determinative factor controlling the expression levels of HrpG. In addition, mutation analysis revealed that the Xoc XrvB also plays positive roles in regulating bacterial growth, cell motility, and stress tolerance. Our findings provide important insights into the molecular mechanism of T3SS expression regulation in Xoc.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台