Identification and Functional Analysis of the Pheromone Response Factor Gene of Sporisorium scitamineum
文献类型： 外文期刊
作者： Zhu, Guining 1 ; Deng, Yizhen 1 ; Cai, Enping 1 ; Yan, Meixin 1 ; Cui, Guobing 1 ; Wang, Zhiqiang 3 ; Zou, Chengwu 3 ; Zh 1 ;
作者机构： 1.South China Agr Univ, Dept Plant Pathol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou, Guangdong, Peoples R China
2.Guangxi Acad Agr Sci, Plant Protect Res Inst, Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning, Peoples R China
3.Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Peoples R China
关键词： Sporisorium scitamineum; pheromone response factor; mating; pathogenicity; fungi
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2019 年 10 卷
页码：
收录情况： SCI
摘要： The sugarcane smut fungus Sporisorium scitamineum is bipolar and produces sporidia of two different mating types. During infection, haploid cells of opposite mating types can fuse to form dikaryotic hyphae that can colonize plant tissue. Mating and filamentation are therefore essential for S. scitamineum pathogenesis. In this study, we obtained one T-DNA insertion mutant disrupted in the gene encoding the pheromone response factor (Prf1), hereinafter named SsPRF1, of S. scitamineum, via Agrobacterium tumefaciens-mediated transformation (ATMT) mutagenesis. Targeted deletion of SsPRF1 resulted in mutants with phenotypes similar to the T-DNA insertion mutant, including failure to mate with a compatible wild-type partner strain and being non-pathogenic on its host sugarcane. qRT-PCR analyses showed that SsPRF1 was essential for the transcription of pheromone-responsive mating type genes of the a1 locus. These results show that SsPRF1 is involved in mating and pathogenicity and plays a key role in pheromone signaling and filamentous growth in S. scitamineum.
- 相关文献
作者其他论文 更多>>
-
Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption
作者：Zhang, Yi;He, Yongding;Zhang, Shilong;Zhou, Sisi;Shen, Wankuan;Zhang, Yi;He, Yongding;Zhang, Shilong;Zhou, Sisi;Shen, Wankuan;Yan, Meixin
关键词：sugarcane; Sporisorium scitamineum; Major Facilitator Superfamily (MFS) sugar transporter; sexual mating; pathogenicity
-
Dual-modal improved biosensing platform for sugarcane smut pathogen based on biological enzyme-Mg2+ DNAzyme coupled with DNA transporter cascading hybridization chain reaction
作者：Che, Rongshuai;Tang, Danyao;Fu, Bingtao;Wu, Yeyu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Yan, Meixin;Ya, Yu
关键词：3D DNA transporter; Sugarcane smut pathogen; Dual-modal detection
-
Development and application of sugarcane yellow leaf virus vectors in sugarcane
作者：Yin, Yuteng;Zhao, Pingzhi;Sun, Yanwei;Han, Tian;Wang, Mengrong;Ye, Jian;Yin, Yuteng;Zhao, Pingzhi;Ye, Jian;Han, Tian;Wang, Mengrong;Yan, Meixin;Wu, Jianmin;Zhou, Hui
关键词：Sugarcane yellow leaf virus; Sugarcane; Aphid; Infectious clone; Expression vector
-
SsCyp86 modulates sporisorium scitamineum mating/filamentation and pathogenicity through regulating fatty acid metabolism
作者：Cai, Enping;Cai, Enping;Feng, Ruqing;Zheng, Wenqiang;Wang, Yifang;Chang, Changqing;Deng, Jiaru;Yan, Meixin
关键词：Sporisorium scitamineum; fatty acid metabolism; cytochrome P450 enzyme SsCyp86; mating/filamentation; pheromone response transcription factor SsPrf1
-
Discovery of N-Benzyl-4-(1-bromonaphthalen-2-yl)oxybutan-1-amine as a Potential Antifungal Agent against Sporidia Growth and Teliospore Germination of Sporisorium scitamineum
作者：Cai, Enping;Zeng, Rong;Feng, Ruqing;Zhang, Li;Li, Lei;Jia, Huan;Zheng, Wenqiang;Chen, Shaofang;Chang, Changqing;Cai, Enping;Zeng, Rong;Feng, Ruqing;Zhang, Li;Li, Lei;Jia, Huan;Zheng, Wenqiang;Chen, Shaofang;Chang, Changqing;Yan, Meixin
关键词：Sporisorium scitamineum; heat shock protein 104; MAP kinase Hog1; N-benzyl-4-(1-bromonaphthalen-2-yl)oxybutan-1-amine
-
Simulating Sugarcane Elongation by a Multi-Parameter Generalized Logistic Model with Meteorological Effect
作者：Zheng, Guojun;Ma, Pengcheng;Wang, Qinlong;Zhu, Guanghu;Huang, Hairong;Luo, Ting;Li, Xiang;Yan, Meixin;Wang, Zeping
关键词：Sugarcane stalk elongation; Meteorological factors; Multi-parameter generalized logistic model; Quantification
-
The Interaction between Hypovirulence-Associated Chrysoviruses and Their Host Fusarium Species
作者：Zou, Chengwu;Cao, Xueying;Zhou, Qiujuan;Yao, Ziting;Yao, Ziting
关键词：chrysovirus; Fusarium; mycovirus-host interactions; viral infection; transmission; RNA interference
