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Overexpression of the peanut CLAVATA1-like leucine-rich repeat receptor-like kinase AhRLK1 confers increased resistance to bacterial wilt in tobacco

文献类型： 外文期刊

作者： Zhang, Chong 1 ; Chen, Hua 1 ; Zhuang, Rui-Rong 1 ; Chen, Yu-Ting 1 ; Deng, Ye 1 ; Cai, Tie-Cheng 1 ; Wang, Shuai-Yin; 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Plant Protect, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Fujian, Peoples R China

2.Fujian Agr & Forestry Univ, Key Lab Crop Mol & Cell Biol, Fuzhou 350002, Fujian, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Resources & Environm, Inst Plant Nutr Physiol & Mol Biol, Fuzhou 350002, Fujian, Peoples R China

4.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Fujian, Peoples R China

5.Guangxi Acad Agr Sci, Cash Crops Res Inst, Nanning 530007, Peoples R China

6.Shandong Peanut Res Inst, Qingdao 266100, Shandong, Peoples R China

7.Natl Tsing Hua Univ, Coll Life Sci, Dept Life Sci, Hsinchu 30013, Taiwan

8.Natl Tsing Hua Univ, Coll Life Sci, Inst Bioinformat & Struct Biol, Hsinchu 30013, Taiwan

关键词： AhRLK1; Arachis hypogaea; defense signaling; EDS1; Ralstonia solanacearum; transgenic tobacco

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2019 年 70 卷 19 期

页码：

收录情况： SCI

摘要： Bacterial wilt caused by Ralstonia solanacearum is a devastating disease affecting hundreds of plant species, yet the host factors remain poorly characterized. The leucine-rich repeat receptor-like kinase gene AhRLK1, characterized as CLAVATA1, was found to be up-regulated in peanut upon inoculation with R. solanacearum. The AhRLK1 protein was localized in the plasma membrane and cell wall. qPCR results showed AhRLK1 was induced in a susceptible variety but little changed in a resistant cultivar after inoculated with R. solanacearum. Hormones such as salicylic acid, abscisic acid, methyl jasmonate, and ethephon induced AhRLK1 expression. In contrast, AhRLK1 expression was down-regulated under cold and drought treatments. Transient overexpression of AhRLK1 led to a hypersensitive response (HR) in Nicotiana benthamiana. Furthermore, AhRLK1 overexpression in tobacco significantly increased the resistance to R. solanacearum. Besides, the transcripts of most representative defense responsive genes in HR and hormone signal pathways were significantly increased in the transgenic lines. EDS1 and PAD4 in the R gene signaling pathway were also up-regulated, but NDR1 was down-regulated. Accordingly, AhRLK1 may increase the defense response to R. solanacearum via HR and hormone defense signaling, in particular through the EDS1 pathway of R gene signaling. These results provide a new understanding of the CLAVATA1 function and will contribute to genetic enhancement of peanut.

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