文献类型： 外文期刊

作者： Tang, Qihe ¹ ; Lu, Tiedong ³ ; Zhang, Junya ¹ ; Huang, Haining ⁴ ; Guo, Bing ⁵ ; Rocha, Ulisses ⁶ ; Nikolausz, Marcell ⁷ ; Shen, Peihong ² ; Wei, Yuansong ¹ ; Deng, Ye ¹ ; Richnow, Hans Hermann ⁸ ;

作者机构： 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Joint Lab Environm Simulat & Pollut Cont, Beijing 100085, Peoples R China

2.Guangxi Univ, Coll Life Sci & Technol, Nanning 530005, Peoples R China

3.Guangxi Acad Agr Sci, Agr Resources & Environm Res Inst, Guangxi Key Lab Arable Land Conservat, Nanning 530007, Guangxi, Peoples R China

4.Tongji Univ, Sch Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China

5.Univ Surrey, Ctr Environm Hlth & Engn CEHE, Dept Civil & Environm Engn, Guildford GU2 7XH, England

6.UFZ Helmholtz Ctr Environm Res, Dept Appl Microbial Ecol, D-04318 Leipzig, Germany

7.UFZ Helmholtz Ctr Environm Res, Dept Microbial Biotechnol, D-04318 Leipzig, Germany

8.UFZ Helmholtz Ctr Environm Res, Dept Tech Biogeochem, Permoserstr, D-04318 Leipzig, Germany

9.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

关键词： Antibiotic resistance genes expression; Anaerobic digestion; Viral community; Metagenomics; Reverse transcription high-throughput quanti-; tative polymerase chain reaction

期刊名称：CHEMICAL ENGINEERING JOURNAL （ 影响因子：13.2； 五年影响因子：13.5 ）

ISSN： 1385-8947

年卷期： 2025 年 509 卷

页码：

收录情况： SCI

摘要： This study investigated the effects of chlortetracycline (CTC) and copper (Cu), individually and in combination, on the transfer and expression of antibiotic resistance genes (ARGs) in anaerobic digestion (AD). Utilizing metagenomics and high-throughput quantitative PCR (HT-qPCR), we found that high concentrations of Cu (400 mgL (-1)) and CTC (80 mgL (-1)) significantly enriched the relative abundance of ARGs attributed to the changes of bacterial community (p < 0.05), whereas lower concentrations (Cu: 40 mgL (-1); CTC: 8 mgL (-1)) had minimal effects. The species abundance distribution and neutral community models indicated that antibiotic resistome is determined by stochastic processes, and the abundance of ARGs is determined by a limited number of core ARGs, showing the resilience to the selection of antibiotics and heavy metals. Reverse transcription HT-qPCR indicated that most ARGs were silent or expressed at low levels; however, regardless of the concentration, CTC enhanced the expression of ARGs, particularly those linked to tetracycline ribosome protection. In contrast, high Cu levels inhibited ARGs expression due to its non-selective toxicity, which was evidenced by a failure to produce methane. CTC and Cu both showed limited impacts on the potential mobility of ARGs shown by metagenomics, although TET significantly increased the conjugation frequency reflected by conjugation assays (p < 0.05). ARGs carried by virus only accounted for 0.26 % +/- 0.10 % of the total, with no evidence of phage-mediated transduction, and phage lysis significantly contributed to ARGs reduction. While CTC and Cu showed limited effects on phage-carrying ARGs, they notably inhibited phage lytic activity, as indicated by virus-host ratios and phage lysis tests, further leading to the enrichment of ARGs in AD system. Our findings provide novel insights into the ARGs transfer under selective pressure of antibiotics and heavy metals in AD.