Viral Communities Contribute More to the Lysis of Antibiotic-Resistant Bacteria than the Transduction of Antibiotic Resistance Genes in Anaerobic Digestion Revealed by Metagenomics
文献类型： 外文期刊
作者： Zhang, Junya 1 ; Lu, Tiedong 4 ; Song, Yunpeng 1 ; Rocha, Ulisses Nunes da 5 ; Liu, Jibao 1 ; Nikolausz, Marcell 5 ; Wei, Yuansong 1 ; Richnow, Hans Hermann 2 ;
作者机构： 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Joint Lab Environm Simulat & Pollut Cont, Beijing 100085, Peoples R China
2.UFZ Helmholtz Ctr Environm Res, Dept Isotope Biogeochem, D-04318 Leipzig, Germany
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Guangxi Acad Agr Sci, Agr Resource & Environm Res Inst, Guangxi Key Laborarory Arable Lnad Conservat, Nanning 530007, Guangxi, Peoples R China
5.UFZ Helmholtz Ctr Environm Res, Dept Environm Microbiol, D-04318 Leipzig, Germany
关键词： phages; anaerobic digestion; antibioticresistancegenes; viral community; metagenomics
期刊名称：ENVIRONMENTAL SCIENCE & TECHNOLOGY （ 影响因子：11.4； 五年影响因子：12.0 ）
ISSN： 0013-936X
年卷期： 2024 年 58 卷 5 期
页码：
收录情况： SCI
摘要： Ecological role of the viral community on the fate of antibiotic resistance genes (ARGs) (reduction vs proliferation) remains unclear in anaerobic digestion (AD). Metagenomics revealed a dominance of Siphoviridae and Podoviridae among 13,895 identified viral operational taxonomic units (vOTUs) within AD, and only 21 of the vOTUs carried ARGs, which only accounted for 0.57 +/- 0.43% of AD antibiotic resistome. Conversely, ARGs locating on plasmids and integrative and conjugative elements accounted for above 61.0%, indicating a substantial potential for conjugation in driving horizontal gene transfer of ARGs within AD. Virus-host prediction based on CRISPR spacer, tRNA, and homology matches indicated that most viruses (80.2%) could not infect across genera. Among 480 high-quality metagenome assembly genomes, 95 carried ARGs and were considered as putative antibiotic-resistant bacteria (pARB). Furthermore, lytic phages of 66 pARBs were identified and devoid of ARGs, and virus/host abundance ratios with an average value of 71.7 indicated extensive viral activity and lysis. The infectivity of lytic phage was also elucidated through laboratory experiments concerning changes of the phage-to-host ratio, pH, and temperature. Although metagenomic evidence for dissemination of ARGs by phage transduction was found, the higher proportion of lytic phages infecting pARBs suggested that the viral community played a greater role in reducing ARB numbers than spreading ARGs in AD.
- 相关文献
作者其他论文 更多>>
-
Response of antibiotic resistance genes expression and mediating role of viral community to antibiotics and heavy metals in anaerobic digestion
作者：Tang, Qihe;Zhang, Junya;Wei, Yuansong;Deng, Ye;Tang, Qihe;Shen, Peihong;Lu, Tiedong;Huang, Haining;Guo, Bing;Rocha, Ulisses;Nikolausz, Marcell;Richnow, Hans Hermann;Zhang, Junya;Wei, Yuansong;Deng, Ye
关键词：Antibiotic resistance genes expression; Anaerobic digestion; Viral community; Metagenomics; Reverse transcription high-throughput quanti-; tative polymerase chain reaction
-
Response of prokaryotic, eukaryotic and algal communities to heavy rainfall in a reservoir supplied with reclaimed water
作者：Xin, Yuan;Shen, Peihong;Xin, Yuan;Zhang, Junya;Wei, Yuansong;Zhang, Junya;Zhang, Junya;Wei, Yuansong;Lu, Tiedong
关键词：Reservoir; Heavy rainfall; Prokaryotic community; Eukaryotic community; Algal community
-
Anaerobic co-digestion of molasses vinasse and three kinds of manure: A comparative study of performance at different mixture ratio and organic loading rate
作者：Qin, Ya;Huang, Luodong;Jiang, Qiong;Xin, Yuan;Liu, Jiantao;Shen, Peihong;Lu, Tiedong;Zhen, Yuantao
关键词：Animal manure; Co-digestion; Organic loading rate; Microbial diversity
-
Coupled mechanism of enhanced and inhibitory effects of nanoscale zero-valent iron on methane production and antibiotic resistance genes in anaerobic digestion of swine manure
作者：Lu, Tiedong;Su, Tianming;He, Tieguang;Zhang, Junya;Zhang, Junya;Zhang, Junya;Wei, Yuansong;Liang, Xuelian
关键词：Swine manure; Nanoscale zero-valent iron; Anaerobic digestion; High-throughput qPCR; Antibiotic resistance genes
-
Dual character of methane production improvement and antibiotic resistance genes reduction by nano-Fe2O3 addition during anaerobic digestion of swine manure
作者：Lu, Tiedong;Wei, Yuansong;Zhang, Junya;Lu, Tiedong;Su, Tianming;He, Tieguang;Zhang, Junya;Wei, Yuansong;Zhang, Junya;Liang, Xuelian
关键词：Swine manure; Anaerobic digestion; Microbial community; Antibiotic resistance genes
-
Ferric chloride further simplified the horizontal gene transfer network of antibiotic resistance genes in anaerobic digestion
作者：Zhang, Junya;Wei, Yuansong;Zhang, Junya;Zhang, Junya;Wei, Yuansong;Lu, Tiedong;Xin, Yuan
关键词：Ferricchloride; Antibioticresistancegenes; Verticalgenetransfer; Horizontalgenetransfer; Anaerobicdigestion
