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The ci-miR-5 regulates the CiCYP18A1 to control moulting process of Chilo infuscatellus: Prospective target for pest control

文献类型： 外文期刊

作者： Wang, Jin-da 1 ; Lin, Huan-tai 1 ; Yang, Zhi-han 1 ; Chen, Li-Fei 1 ; Tong, Yi-lu 1 ; Wei, Ji-li 2 ; Pan, Xue-hong 2 ; Shang, Xian-kun 2 ; Gao, San-ji 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Natl Engn Res Ctr Sugarcane, State Key Lab Agr & Forestry Biosecur, Fuzhou, Peoples R China

2.Guangxi Acad Agr Sci, Key Lab Sugarcane Biotechnol & Genet Improvement G, Guangxi Key Lab Sugarcane Genet Improvement, Sugarcane Res Inst,Minist Agr & Rural Affairs, Nanning, Peoples R China

关键词： Chilo infuscatellus; ecdysone metabolism; miRNA regulation; moult

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.3； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2025 年

页码：

收录情况： SCI

摘要： The microRNAs (miRNAs) play an important role in the regulation of gene expression, influencing the outcome of many biological processes in insects. The introduction of miRNA into the insect body can lead to pest developmental abnormalities indicating that miRNAs are potential targets for pest control. In this study, we employed small RNA sequencing to characterise and analyse the expression of miRNAs in the sugarcane shoot borer Chilo infuscatellus (Snellen). By integrating previously published transcriptome sequencing data, we predicted and identified miRNAs and their target genes involved in the ecdysone metabolic pathway. We confirmed the target relationships between Ci-miR-4, Ci-miR-5 and CiCYP18A1 by RT-qPCR and dual-luciferase reporter assays in vivo and in vitro. Additionally, injection of miRNA mimics reduced the transcripts of CiCYP18A1 and led to abnormal moulting, which is similar to the defect phenotype of depletion of CiCYP18A1 by dsRNA. Furthermore, we also mixed the synthesised Ci-miR-5 mimic into artificial diets and found similar stunting results of the injection method. These results demonstrated that Ci-miR-5 could target CiCYP18A1 to regulate the moulting process and the successful use of an insect miRNA for gene silencing studies in this sugarcane pest. The method provided an effective novel approach for future RNAi-based pest control strategies.

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