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Comparison of leaf transcriptomes of cassava "Xinxuan 048" diploid and autotetraploid plants

文献类型： 外文期刊

作者： Yin, Ling 1 ; Qu, Junjie 1 ; Zhou, Huiwen 1 ; Shang, Xiaohong 1 ; Fang, Hui 1 ; Lu, Jiang 1 ; Yan, Huabing 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China

2.Shanghai Jiao Tong Univ, Sch Agr & Biol, Ctr Viticulture & Enol, Shanghai 200024, Peoples R China

关键词： Autotetraploid; Cassava; Morphology; Physiology; Transcriptome

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN： 1976-9571

年卷期： 2018 年 40 卷 9 期

页码：

收录情况： SCI

摘要： Polyploidy breeding of cassava has been used to improve cassava traits over the past years. We previously reported in vitro induction of tetraploids in the cassava variety "Xinxuan 048" using colchicine. Significant differences in morphology and anatomy were found between the diploid and tetraploid plants. However, very little is known about the transcriptome difference between them. In this study, morphological and physiological characteristics including leaf thickness, plant height, internode length, chlorophyll content, and photosynthetic capacity were measured. Further, we investigated and validated the difference in gene expression patterns between cassava "Xinxuan 048" tetraploid genotype and its diploid plants using RNA sequencing (RNAseq) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Significant differences in morphology and physiology were observed during tetraploidization. A comparison revealed that tetraploidy induced very limited changes in the leaf transcriptomes of cassava "Xinxuan 048" diploid and autotetraploid plants. However, the differentially expressed genes (DEGs) between 2x and 4x plants, especially those upregulated in 4x plants, were strongly associated with hormonal and stress responses. Large changes in morphology and physiology between the diploid cassava "Xinxuan 048" and its autotetraploid were not associated with large changes in their leaf transcriptomes. Moreover, the differently expressed genes related to the regulation of gibberellin and brassinosteroids potentially explained why the plant height and internode length of 4x plants became shorter. Collectively, our results suggest that 4x cassava is potentially valuable for breeding strains with improved stress resistance.

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