RPW8 promoter is involved in pathogen-And stress-inducible expression from Vitis pseudoreticulata
文献类型： 外文期刊
作者： Lai, Gongti 2 ; Song, Shiren 2 ; Liu, Yunxiao 1 ; Fu, Peining 1 ; Xiang, Jiang 2 ; Lu, Jiang 2 ;
作者机构： 1.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Agr & Biol, Ctr Viticulture & Enol, Shanghai, Peoples R China
3.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning, Peoples R China
关键词： Phytophthora capsici; Plasmopara viticola; RPW8 promoter; salicylic acid; Vitis pseudoreticulata
期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.789； 五年影响因子：1.574 ）
ISSN： 0931-1785
年卷期： 2019 年 167 卷 2 期
页码：
收录情况： SCI
摘要： Based on previous cloning of VpRPW8-e, we obtained a 1,126bp VpRPW8-e promoter sequence in this study. A large number of TATA-boxes, CAAT-boxes, and other cis-acting elements were predicted including light-responsive elements, hormone-responsive elements, stress-responsive elements, and growth- and development-associated elements within the promoter sequence. To further investigate the function of this promoter, we examined its activity in response to biotic and abiotic stress. The VpRPW8-e promoter was strongly activated by Plasmopara viticola infection, and activation also occurred when the orientation of the promoter was reversed, although to a lesser extent. Deletion analysis showed that the -1,126 to -475 bp region of VpRPW8-e promoter had high activity. A promoter fragment 5 deleted to -475 bp (P-475) was activated in response to heat and cold stress, and even more strongly in response to Phytophthora capsici and salicylic acid (SA). Furthermore, Transgenic Nicotiana benthamiana were generated, VpRPW8-e driven by P-475 enhanced resistance to Ph. capsici in N. benthamiana. Based on these results, the -475 bp region was deduced to be an indispensable part of the VpRPW8-e promoter. VpRPW8-e promoter is involved in pathogen- and stress-inducible expression.
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