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Identifying Plasmopara viticola resistance Loci in grapevine (Vitis amurensis) via genotyping-by-sequencing-based QTL mapping

文献类型： 外文期刊

作者： Fu, Peining 1 ; Wu, Wei 1 ; Lai, Gongti 2 ; Li, Rongfang 1 ; Peng, Yachun 1 ; Yang, Bohan 1 ; Wang, Bo 1 ; Yin, Ling 3 ; Qu, 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Ctr Viticulture & Enol, Sch Agr & Biol, Shanghai, Peoples R China

2.Fujian Acad Agr Sci, Inst Agr Engn & Technol, Fuzhou, Fujian, Peoples R China

3.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning, Guangxi, Peoples R China

关键词： Grapevine; Vitis amurensis; Plasmopara viticola resistance; Genotyping-by-sequencing; Quantitative trait locus; Mapping

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 154 卷

页码：

收录情况： SCI

摘要： Downy mildew, caused by Plasmopara viticola, is a major disease that affects grapevines, and a few resistance (R) genes have been identified thus far. In order to identify R genes, we investigated F-1 progeny from a cross between the downy mildew-resistant Vitis amurensis 'Shuang Hong' and the susceptible Vitis vinifera 'Cabernet Sauvignon'. The P. viticola-resistance of the progeny varied continuously and was segregated as a quantitative trait. Genotyping-by-sequencing was used to construct linkage maps. The integrated map spanned 1898.09 cM and included 5603 single nucleotide polymorphisms on 19 linkage groups (LGs). Linkage analysis identified three quantitative trait loci (QTLs) for P. viticola resistance: 22 (Rpv22) on LG 02, Rpv23 on LG15, and Rpv24 on LG18. The phenotypic variance contributed by these three QTLs ranged from 15.9 to 30.0%. qRT-PCR analysis of R-gene expression in these QTLs revealed a CC-NBS-LRR disease resistance gene RPP8, two LRR receptor-like serine/threonine-protein kinases, a serine/threonine-protein kinase BLUS1, a glutathione peroxidase 8, an ethylene-responsive transcription factor ERF038, and a transcription factor bZIP11 were induced by P. viticola, and these genes may play important role in P. viticola response.

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