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Simple and sensitive detection of triazophos pesticide by using quantum dots nanobeads based on immunoassay

文献类型： 外文期刊

作者： Liao, Yun 1 ; Cui, Xueyan 2 ; Chen, Ge 2 ; Wang, Yuanshang 2 ; Qin, Guoxin 3 ; Li, Mingjie 2 ; Zhang, Xiuyuan 2 ; Zhang, 1 ;

作者机构： 1.Hainan Univ, Coll Food Sci & Technol, Haikou 570228, Hainan, Peoples R China

2.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Qual & Safety, Beijing, Peoples R China

3.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning, Peoples R China

4.Cairo Univ, Dept Pharmacol, Fac Vet Med, Giza, Egypt

关键词： Immunoassay; fluorescence; quantum dots; antibody

期刊名称：FOOD AND AGRICULTURAL IMMUNOLOGY （ 影响因子：3.101； 五年影响因子：2.573 ）

ISSN： 0954-0105

年卷期： 2019 年 30 卷 1 期

页码：

收录情况： SCI

摘要： A rapid, ultra-sensitive and simple fluorescence immunoassay (FLISA) on CdSe/ZnS Quantum dots (QDs) for recognition pesticide triazophos have been established. In this assay, the immunoassay uses (CdSe/ZnS) Quantum dots (QDs) as a probe that carries monoclonal antibody (mAb). The free triazophos and OVA-haptens were competed for binding the mAb on the surface of the QDs. Therefore, the concentration of TRIAZ can get by calculation of the detection of fluorescence value. The assay has a linear response in the 10 ng L-1-25 mu g L-1 TRIAZ concentration range, and limit of detection (IC10) was 0.508 ng L-1. The recovery rate in the case of spiked samples ranges from 82.6% to 96.6%, and the RSD is <20%. FLISA can accurately and sensitively screen out triazophos in representative actual samples that contain trace levels of pesticides, it can play a prominent part in agricultural product and environmental detection.

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