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The Farnesyltransferase beta-Subunit Ram1 Regulates Sporisorium scitamineum Mating, Pathogenicity and Cell Wall Integrity

文献类型： 外文期刊

作者： Sun, Shuquan 1 ; Deng, Yizhen 1 ; Cai, Enping 1 ; Yang, Meixin 2 ; Li, Lingyu 1 ; Chen, Baoshan 3 ; Chang, Changqing 1 ;

作者机构： 1.South China Agr Univ, Coll Agr, Guangdong Prov Key Lab Microbial Signals & Dis Co, Guangzhou, Guangdong, Peoples R China

2.Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning, Peoples R China

3.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Peoples R China

关键词： cell wall integrity; mating; RAM1; Ras; Sporisorium scitamineum

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： The basidiomycetous fungus Sporisorium scitamineum causes a serious sugarcane smut disease in major sugarcane growing areas. Sexual mating is essential for infection to the host; however, its underlying molecular mechanism has not been fully studied. In this study, we identified a conserved farnesyltransferase (FTase) beta subunit Ram1 in S. scitamineum. The ram1 Delta mutant displayed significantly reduced mating/filamentation, thus of weak pathogenicity to the host cane. The ram1 Delta mutant sporidia showed more tolerant toward cell wall stressor Congo red compared to that of the wild-type. Transcriptional profiling showed that Congo red treatment resulted in notable up-regulation of the core genes involving in cell wall integrity pathway in ram1 Delta sporidia compared with that of WT, indicating that Ram1 may be involved in cell wall integrity regulation. In yeast the heterodimeric FTase is responsible for post-translational modification of Ras (small G protein) and a-factor (pheromone). We also identified and characterized two conserved Ras proteins, Ras1 and Ras2, respectively, and a MAT-1 pheromone precursor Mfa1. The ras1 Delta, ras2 Delta and mfa1 Delta mutants all displayed reduced mating/filamentation similar as the ram1 Delta mutant. However, both ras1 Delta and ras2 Delta mutants were hypersensitive to Congo red while the mfa1 Delta mutant was the same as wild-type. Overall our study displayed that RAM1 plays an essential role in S. scitamineum mating/filamentation, pathogenicity, and cell wall stability.

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