Colorimetric bio-barcode immunoassay for parathion based on amplification by using platinum nanoparticles acting as a nanozyme
文献类型： 外文期刊
作者： Chen, Ge 2 ; Jin, Maojun 1 ; Yan, Mengmeng 1 ; Cui, Xueyan 1 ; Wang, Yuanshang 1 ; Zheng, Weijia 1 ; Qin, Guoxin 3 ; Zhan 1 ;
作者机构： 1.Chinese Acad Agicultural Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Minist Agr, Key Lab Agroprod Qual & Safety, Beijing 100081, Peoples R China
3.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning 530007, Peoples R China
4.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
5.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkey
关键词： AuNPs; PtNPs; Pesticide; Colorimetric detection; Nanomaterial; Agro-products; DNA; Magnetic nanoparticles
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
ISSN： 0026-3672
年卷期： 2019 年 186 卷 6 期
页码：
收录情况： SCI
摘要： A competitive bio-barcode immunoassay is described for the trace detection of parathion in water, pear, cabbage, and rice samples. It is based on amplification by platinum nanoparticle acting as a nanozyme. Gold nanoparticles (AuNPs) were modified with (a) monoclonal antibodies (mAbs) against parathion, and (b) thiolated single-stranded DNA (ssDNA) oligonucleotides. Magnetic nanoparticles (MNPs) were functionalized with ovalbumin coupled with parathion hapten. Parathion and its hapten compete with mAbs on the surface of the AuNPs. Subsequently, the platinum nanoparticles (PtNPs) probe, which was functionalized with the complementary thiolated ssDNA (C-ssDNA), was added to the reaction mixture for the detection of parathion. The signal was catalytically amplified by coupling with platinum nanozyme using teramethylbenzidine and H2O2 as the chromogenic system. The immunoassay has a linear range that extends from 0.01-50gL(-1), and the limit of detection is 2.0x10(-3)gL(-1). The recoveries and relative standard deviations (RSDs) ranged from 91.1-114.4% and 3.6-15.8%, respectively. The method correlates well with data obtained by gas chromatography-tandem mass spectrometry (GC-MS/MS).
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