Unraveling the mechanisms of multiple resistance across glyphosate and glufosinate in Eleusine indica
文献类型： 外文期刊
作者： Chen, Jingchao 1 ; Shan, Bin 2 ; Li, Zhiling 1 ; Chen, Qian 3 ; Yu, Haiyan 1 ; Cui, Hailan 1 ; Li, Xiangju 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China
2.Guangxi Subtrop Crops Res Inst, Nanning 530001, Guangxi, Peoples R China
3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China
关键词： Goosegrass; EPSPS; Glutamine synthetase; Copy number variation; Herbicide resistance; Overexpression
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）
ISSN： 0048-3575
年卷期： 2024 年 206 卷
页码：
收录情况： SCI
摘要： The herbicides glyphosate and glufosinate are commonly used in citrus and sugarcane orchards in Guangxi Province, China, wherein the C4 plant Eleusine indica (L.) Gaertn. is known to be a dominant weed species. However, high selection pressure has resulted in failure of control. In the present study, experiments were conducted to clarify resistance levels for the suspected populations and elucidate the mechanisms for multiple resistance. The resistance index to glyphosate was calculated for eight populations and ranged from 5.4 to 21.3, with a low-level shikimate content of 0.24-0.50 mu g g- 1. In addition, three populations showed low-level resistance to glufosinate, with a resistance index ranging from 2.6 to 3.9. The amplification of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene with a double-mutation T102I + P106S (TIPS) or a single-mutation (P106S and P106A) was observed in most populations. The target genes of glufosinate (GS1-1, GS1-2, and GS1-3) showed high-level expression, namely 145.5-fold that of susceptible plants. The content of EPSPS and glutamine synthetase (GS) protein in resistant plants can reach to 3.6 and 2.1 times higher than those in susceptible plants, respectively. The overexpression of the EPSPS gene with double (T102I + P106S) or single (P106S and P106A) mutations, plus the overexpression of GS1-1, GS1-2, and GS1-3, responded to multiple resistance mechanisms. Altogether, these results demonstrate that overexpression of GS1 is a novel form of resistant mechanism to glufosinate in weeds.
- 相关文献
作者其他论文 更多>>
-
A Rapid PCR-LAMP Assay for the Early Detection of Lasiodiplodia theobromae from Basal Stem Rot-Infected Passion Fruit Plants
作者：Liu, Ying;Rasheed, Usman;Shan, Bin;Lu, Qinyu;Chen, Shimiao;Meng, Kaikai;Mo, Ganhui;Liu, Ying;Rasheed, Usman;Shan, Bin;Lu, Qinyu;Chen, Shimiao;Meng, Kaikai;Mo, Ganhui;Qin, Aiying
关键词：Passion fruit; Basal stem rot; Loop-mediated isothermal amplification; Hydroxy naphthol blue; Phytopathogen detection;
Lasiodiplodia theobromae -
First Report of Brown Stripe Disease Caused by Paracidovorax oryzae on Rice in Guangxi (China)
作者：Huang, Jingjing;Wang, Hao;Zeng, Yingyu;Guo, Weiyuan;Yang, Shanshan;Zhang, Xiaoxiao;Li, Fuxing;Pan, Lingling;Shan, Bin;Fei, Nuoya
关键词：brown stripe disease; Paracidovorax oryzae; rice
-
Influence of photoperiod on endogenous phytohormone levels and cadmium-related gene expression in Sedum alfredii
作者：Chen, ShiMiao;Shan, Bin;Lu, QinYu;Chen, ShiMiao;Shan, Bin;Lu, QinYu;Chen, ShiMiao;Lu, QinYu;Zheng, Fuhai;Li, Yanyan
关键词：Photoperiod; Sedum alfredii; phytohormones; cadmium; phytoremediation optimization
-
Enhanced antifungal activity of chitosan-coated cinnamaldehyde-immobilized thermostable cellulose from spent mushroom substrate against phytopathogenic fungi
作者：Rasheed, Usman;Mo, Ganhui;Shan, Bin;Meng, Kaikai;Chen, Ganlin;Rasheed, Usman;Mo, Ganhui;Shan, Bin;Meng, Kaikai;Chen, Ganlin;Rasheed, Usman;Mo, Ganhui;Shan, Bin;Meng, Kaikai;Chen, Ganlin;Liu, Bin;Ul Ain, Qurat
关键词：Cellulose; Cinnamaldehyde; Antifungal; Peanuts; Passion fruit
-
Interactive Effects of Selenium and Arsenic on their Accumulation, Translocation, Arsenic and Selenium Species, and Subcellular Distribution in Brassica Vegetables
作者：Qin, Yuyan;Wang, Yunru;Shi, Pengtao;Wu, Feng;Zhong, Yunjie;Li, Hong;Shan, Bin;Qin, Yuyan;Wang, Yunru;Shi, Pengtao;Wu, Feng;Li, Hong;Shan, Bin;Qin, Yuyan;Wang, Yunru;Shi, Pengtao;Wu, Feng;Zhong, Yunjie;Li, Hong;Shan, Bin
关键词：Arsenic; Selenium; Accumulation; Speciation; Subcellular distribution; Brassica vegetable
-
Identification of Meloidogyne panyuensis (Nematoda: Meloidogynidae) infecting Orah (Citrus reticulata Blanco) and its impact on rhizosphere microbial dynamics: Guangxi, China
作者：Zhang, Xiaoxiao;Zhao, Wei;Lin, Yuming;Yang, Shanshan;Shan, Bin
关键词：Citrus reticulata Blanco; Root-knot nematode; Meloidogyne panyuensis; Rhizosphere soil; Microbiome
