文献类型： 外文期刊

作者： Malviya, Mukesh Kumar ¹ ; Li, Chang-Ning ¹ ; Lakshmanan, Prakash ¹ ; Solanki, Manoj Kumar ⁴ ; Wang, Zhen ⁵ ; Solanki, Anjali Chandrol ⁶ ; Nong, Qian ¹ ; Verma, Krishan K. ¹ ; Singh, Rajesh Kumar ¹ ; Singh, Pratiksha ¹ ; Sharma, Anjney ¹ ; Guo, Dao-Jun ¹ ; Dessoky, Eldessoky S. ⁸ ; Song, Xiu-Peng ¹ ; Li, Yang-Rui ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Guangxi Acad Agr Sci,Sugarcane Res Ctr,Guangxi Ke, Minist Agr & Rural Affairs,Sugarcane Res Inst, Key Lab Sugarcane Biotechnol & Genet Improvement, Nanning, Peoples R China

2.Southwest Univ, Coll Resources & Environm, Interdisciplinary Res Ctr Agr Green Dev Yangtze R, Chongqing, Peoples R China

3.Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld, Australia

4.Univ Silesia Katowice, Inst Biol Biotechnol & Environm Protect, Plant Cytogenet & Mol Biol Grp, Fac Nat Sci, Katowice, Poland

5.Yulin Normal Univ, Coll Biol & Pharm, Guangxi Key Lab Agr Resources Chem & Biotechnol, Yulin, Peoples R China

6.Mansarovar Global Univ, Dept Agr Sci, Bhopal, India

7.Guangxi Univ, Coll Agr, Nanning, Peoples R China

8.Taif Univ, Dept Biol, Coll Sci, At Taif, Saudi Arabia

关键词： rhizosphere soil; sugarcane; nifH; 16S rRNA; diazotroph diversity

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Considering the significant role of genetic background in plant-microbe interactions and that most crop rhizospheric microbial research was focused on cultivars, understanding the diversity of root-associated microbiomes in wild progenitors and closely related crossable species may help to breed better cultivars. This study is aimed to fill a critical knowledge gap on rhizosphere and diazotroph bacterial diversity in the wild progenitors of sugarcane, the essential sugar and the second largest bioenergy crop globally. Using a high-throughput sequencing (HTS) platform, we studied the rhizosphere and diazotroph bacterial community of Saccharum officinarum L. cv. Badila (BRS), Saccharum barberi (S. barberi) Jesw. cv Pansahi (PRS), Saccharum robustum [S. robustum; (RRS), Saccharum spontaneum (S. spontaneum); SRS], and Saccharum sinense (S. sinense) Roxb. cv Uba (URS) by sequencing their 16S rRNA and nifH genes. HTS results revealed that a total of 6,202 bacteria-specific operational taxonomic units (OTUs) were identified, that were distributed as 107 bacterial groups. Out of that, 31 rhizobacterial families are commonly spread in all five species. With respect to nifH gene, S. barberi and S. spontaneum recorded the highest and lowest number of OTUs, respectively. These results were validated by quantitative PCR analysis of both genes. A total of 1,099 OTUs were identified for diazotrophs with a core microbiome of 9 families distributed among all the sugarcane species. The core microbiomes were spread across 20 genera. The increased microbial diversity in the rhizosphere was mainly due to soil physiochemical properties. Most of the genera of rhizobacteria and diazotrophs showed a positive correlation, and few genera negatively correlated with the soil properties. The results showed that sizeable rhizospheric diversity exists across progenitors and close relatives. Still, incidentally, the rhizosphere microbial abundance of progenitors of modern sugarcane was at the lower end of the spectrum, indicating the prospect of Saccharum species introgression breeding may further improve nutrient use and disease and stress tolerance of commercial sugarcane. The considerable variation for rhizosphere microbiome seen in Saccharum species also provides a knowledge base and an experimental system for studying the evolution of rhizobacteria-host plant association during crop domestication.