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Improving the thermostability of alginate lyase FlAlyA with high expression by computer-aided rational design for industrial preparation of alginate oligosaccharides

文献类型： 外文期刊

作者： Zhang, Xiu 1 ; Li, Wei 3 ; Pan, Lixia 2 ; Yang, Liyan 2 ; Li, Hongliang 2 ; Ji, Feng 4 ; Zhang, Yunkai 1 ; Tang, Hongzhen 5 ; Yang, Dengfeng 2 ;

作者机构： 1.Guangxi Univ, Coll Life Sci & Technol, Nanning, Guangxi, Peoples R China

2.Guangxi Acad Sci, Guangxi Beibu Gulf Marine Res Ctr, Natl Engn Res Ctr Nonfood Biorefinery, Guangxi Key Lab Marine Nat Prod & Combinatorial Bi, Nanning, Guangxi, Peoples R China

3.Guangxi Acad Agr Sci, Viticulture & Wine Res Inst, Nanning, Guangxi, Peoples R China

4.Guangxi Acad Sci, Inst Med & Hlth Res, Nanning, Guangxi, Peoples R China

5.Guangxi Univ Chinese Med, Sch Pharm, Nanning, Peoples R China

关键词： alginate lyase; high-level expression; thermostability; rational design; molecular dynamics simulation; alginate oligosaccharide

期刊名称：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY （ 影响因子：6.064； 五年影响因子：6.303 ）

ISSN： 2296-4185

年卷期： 2022 年 10 卷

页码：

收录情况： SCI

摘要： FlAlyA, a PL7 alginate lyase with industrial potential, is widely applied in the preparation the alginate oligosaccharide because of its high activity of degradation the alginate. However, heat inactivation still limits the industrial application of FlAlyA. To further enhance its thermostability, a group of mutants were designed, according to evaluating the B-factor value and free energy change via computer-aided calculation. 25 single-point mutants and one double-points mutant were carried out by site-directed mutagenesis. The optimal two single-point mutants H176D and H71K showed 1.20 and 0.3? increases in the values of T (m), while 7.58 and 1.73 min increases in the values of half-life (t (1/2)) at 50?, respectively, compared with that of the wild-type enzyme. Interestingly, H71K exhibits the comprehensive improvement than WT, including expression level, thermal stability and specific activity. In addition, the mechanism of these two mutants is speculated by multiple sequence alignment, structural basis and molecular dynamics simulation, which is likely to be involved in the formation of new hydrogen bonds and decrease the SASA of the mutants. These results indicate that B-factor is an efficient approach to improves the thermostability of alginate lyase composed of beta-sheet unit. Furthermore, the highest yield of the mutant reached about 650 mg/L, which was nearly 36 times that of previous studies. The high expression, excellent activity and good thermal stability make FlAlyA a potential candidate for the industrial production of alginate oligosaccharides.

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