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Acidovorax citrulli type III effector AopU interferes with plant immune responses and interacts with a watermelon E3 ubiquitin ligase

文献类型： 外文期刊

作者： Yang, Linlin 1 ; Zhao, Mei 3 ; Zhang, Xiaoxiao 2 ; Jiang, Jie 2 ; Fei, Nuoya 1 ; Ji, Weiqin 2 ; Ye, Yunfeng 4 ; Guan, Wei 2 ; Yang, Yuwen 2 ; Zhao, Tingchang 2 ;

作者机构： 1.Shenyang Agr Univ, Plant Protect Coll, Dept Plant Pathol, Shenyang, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing, Peoples R China

3.China Agr Univ, Coll Plant Protect, Dept Plant Pathol, Beijing, Peoples R China

4.Guangxi Acad Agr Sci, Hort Res Inst, Nanning, Guangxi, Peoples R China

5.Chinese Acad Agr Sci, Natl Nanfan Res Inst Sanya, Sanya, Peoples R China

关键词： Acidovorax citrulli; type III effector; AopU; E3 ubiquitin ligase; plant immunity

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Acidovorax citrulli is a seed-borne bacterium that causes bacterial fruit blotch of watermelon and other cucurbit plants worldwide. It uses a type III secretion system to inject type III effectors (T3Es) into plant cells, which affect the host immune responses and facilitate pathogen colonization. However, the current understanding of the specific molecular mechanisms and targets of these effectors in A. citrulli is limited. In this study, we characterized a novel T3E called AopU in A. citrulli group II strain Aac5, which shares homology with XopU in Xanthomonas oryzae. The Agrobacterium-mediated gene transient expression system was used to study the effect of AopU on host immunity. The results showed that AopU localized on the cell membrane and nucleus of Nicotiana benthamiana, inhibited reactive oxygen species burst induced by flg22 and the expression of marker genes associated with pathogen-associated molecular pattern-triggered immunity, but activated salicylic acid and jasmonic acid signal pathways. Further investigations revealed that AopU interacts with E3 ubiquitin ligase ClE3R in watermelon, both in vitro and in vivo. Interestingly, the deletion of aopU did not affect the virulence of A. citrulli, suggesting that AopU may have functional redundancy with other effectors in terms of its role in virulence. Collectively, these findings provide new insights into the mechanism of plant immune responses regulated by A. citrulli T3Es.

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