Characterization of Dof Transcription Factors and the Heat-Tolerant Function of PeDof-11 in Passion Fruit (Passiflora edulis)
文献类型： 外文期刊
作者： Chen, Ge 1 ; Xu, Yi 2 ; Gui, Jie 1 ; Huang, Yongcai 1 ; Ma, Funing 2 ; Wu, Wenhua 2 ; Han, Te 2 ; Qiu, Wenwu 1 ; Yang, Liu 1 ; Song, Shun 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China
2.Natl Key Lab Trop Crop Breeding, Trop Crops Genet Resources Inst, CATAS Germplasm Repository Passiflora, Haikou 571101, Peoples R China
3.Nanjing Agr Univ, Coll Hort, Nanjing 210018, Peoples R China
4.Sanya Res Inst, Hainan Key Lab Biosafety Monitoring & Mol Breeding, CATAS, Sanya 572000, Peoples R China
关键词： Dof transcription factor; passion fruit; high-temperature stress; gene expression; transgenic
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 15 期
页码：
收录情况： SCI
摘要： Abiotic stress is the focus of passion fruit research since it harms the industry, in which high temperature is an important influencing factor. Dof transcription factors (TFs) act as essential regulators in stress conditions. TFs can protect against abiotic stress via a variety of biological processes. There is yet to be published a systematic study of the Dof (PeDof) family of passion fruit. This study discovered 13 PeDof family members by using high-quality genomes, and the members of this characterization were identified by bioinformatics. Transcriptome sequencing and qRT-PCR were used to analyze the induced expression of PeDofs under high-temperature stress during three periods, in which PeDof-11 was significantly induced with high expression. PeDof-11 was then chosen and converted into yeast, tobacco, and Arabidopsis, with the findings demonstrating that PeDof-11 could significantly respond to high-temperature stress. This research lays the groundwork for a better understanding of PeDof gene regulation under high-temperature stress.
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