CLONING, SEQUENCE ANALYSIS, AND FUNCTIONAL STUDY OF THE PEDOF-4 GENE IN RESPONSE TO DROUGHT STRESS
文献类型： 外文期刊
作者： Chen, Ge 1 ; Song, Shun 1 ; Huang, Yongcai 1 ; Jiang, Ping 1 ; Gui, Jie 1 ; Jiang, Xiongying 4 ; Ma, Chonglie 1 ; Mo, Haolan 4 ; Zhou, Bin 4 ; Fan, Dayong 4 ; Yang, Liu 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Key Lab Pass fruit Biol & Genet Breeding, Guangxi Crop Genet Improvement & Biotechnol Lab, Minist Agr & Rural Affairs, Nanning 530007, Peoples R China
2.Trop Crops Genet Resources Inst, Natl Key Lab Trop Crop Breeding, Germplasm Repository Passiflora, CATAS, Haikou 571101, Peoples R China
3.Sanya Res Inst, CATAS, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya 572000, Peoples R China
4.Guilin Acad Agr Sci, Guilin 541000, Peoples R China
关键词： Passion fruit; Dof; Sequence analysis; Cloning; Drought
期刊名称：BANGLADESH JOURNAL OF BOTANY （ 影响因子：0.3； 五年影响因子：0.4 ）
ISSN： 0253-5416
年卷期： 2024 年 53 卷 3 期
页码：
收录情况： SCI
摘要： DNA-binding with one finger (Dof) was a functionally important gene in plant stress tolerance processes. The cloned full-length (849 bp) passion fruit PeDof-4 gene was sequenced, which encodes 282 amino acids with the molecular formula C1274H1983N375O408S14, molecular weight of 29529.87 u, and theoretical isoelectric point pI of 8.96. Evolutionary tree analysis showed that the PeDof-4-encoded proteins had a high degree of identity with Dof-encoded proteins in other plants. Blastp of the PeDof-4 encoded proteins revealed a closer affinity to the proteins encoded by the Dof of Theobro ma cacao. The relative expression of the PeDof-4 gene was higher at 50% of the relative soil moisture content in the fluorescence qPCR experiments under different levels of drought stress. Tissue-specific analysis showed that the highest expression of this gene was found in the roots. Overexpression of GUS gene under the PeDof-4 promoter in Arabidopsis thaliana showed that PeDof-4 could significantly respond to drought stress.
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