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Identification of a new allele of soluble starch synthase IIIa involved in the elongation of amylopectin long chains in a chalky rice mutant

文献类型： 外文期刊

作者： Ying, Yining 1 ; Hu, Yaqi 1 ; Zhang, Yanni 1 ; Tappiban, Piengtawan 1 ; Zhang, Zhongwei 1 ; Dai, Gaoxing 3 ; Deng, Guofu 3 ; Bao, Jinsong 1 ; Xu, Feifei 1 ;

作者机构： 1.Zhejiang Univ, Inst Nucl Agr Sci, Coll Agr & Biotechnol, Key Lab Nucl Agr Sci,Minist Agr & Zhejiang Prov, Zijingang Campus, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, Yazhou Bay Sci & Technol City, Hainan Inst, Sanya 572025, Peoples R China

3.Guangxi Acad Agr Sci, Rice Res Inst, Nanning 530007, Peoples R China

关键词： Rice; Chalky mutant; Soluble starch synthase; Starch synthesis; Rice grain quality

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2023 年 328 卷

页码：

收录情况： SCI

摘要： A chalky endosperm mutant (GM03) induced from an indica rice GLA4 was used to investigate the functional gene in starch biosynthesis. Bulked segregant analysis and sanger sequencing determined that a novel mutation in soluble starch synthase IIIa (SSIIIa) is responsible for the chalky phenotype in GM03. Complementary test by transforming the active SSIIIa gene driven by its native promoter to GM03 recovered the phenotype to its wildtype. The expression of SSIIIa was significantly decreased, while SSIIIa protein was not detected in GM03. The mutation of SSIIIa led to increased expression of most of starch synthesis related genes and elevated the levels of most of proteins in GM03. The CRISPR/Cas9 technology was used for targeted disruption of SSIIIa, and the mutant lines exhibited chalky endosperm which phenocopied the GM03. Additionally, the starch fine structure in the knockout mutant lines ss3a-1 and ss3a-2 was similar with the GM03, which showed increased amylose content, higher proportions of B1 and B2 chains, much lower proportions of B3 chains and decreased degree of crystallinity, leading to altered thermal properties with lower gelatinization temperature and enthalpy. Collectively, these results suggested that SSIIIa plays an important role in starch synthesis by elongating amylopectin long chains in rice.

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