Hydrophobic-flexible rational modification strategy provides fructooligosaccharides activity from Aspergillus niger
文献类型： 外文期刊
作者： Chen, Gan-Lin 1 ; Chen, Jing 2 ; Zhao, Ling-Zhi 4 ; Lin, Bo 5 ; Zheng, Feng-Jin 5 ; Verma, Krishan K. 6 ; Yang, Li-Fang 1 ;
作者机构： 1.Guangxi Minzu Univ, Sch Chem & Chem Engn, Nanning 530006, Guangxi, Peoples R China
2.Guangxi Acad Agr Sci, Guangxi Subtrop Crops Res Inst, Nanning 530001, Guangxi, Peoples R China
3.Minist Agr & Rural Affairs, Guangxi Key Lab Qual & Safety Control Subtrop, Key Lab Qual & Safety Control Subtrop Fruit &, Nanning 530001, Guangxi, Peoples R China
4.Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Guangxi, Peoples R China
5.Guangxi Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Nanning 530007, Guangxi, Peoples R China
6.Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Guangxi, Peoples R China
关键词： Fructosyltransferase; Fructooligosaccharide; Molecular docking; Structural analysis; Aspergillus niger
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）
ISSN： 0023-6438
年卷期： 2025 年 230 卷
页码：
收录情况： SCI
摘要： Fructooligosaccharides (FOS), linear fructose oligomers with health benefits, are synthesized through sucrose transfructosylation using fructosyltransferases (FTases). This study employed the computer-aided design to modify the hydrophobic-flexible active domain of Aspergillus niger FTases. Mutant 142P exhibited 22.56 % (45.55 mu kat) higher than wild-type FTases, with optimal conditions, i.e, 55 degrees C, pH 5.0, and CO2+. Kinetic analysis reduced Km value (6.174 +/- 0.15 M), attributed to weakened active domain-sucrose interactions. FOS conversion by 142P enhanced up to 0.17-29.20 %, yielding 14.92 % 1-Kestose, and 14.27 % Nystose. Molecular docking enhanced active domain flexibility (structural shift from green to red) and reduced glucose affinity as key factors boosting activity and FOS conversion. Hydrophobic-flexible modification proves effective for optimizing FTases in industrial applications. This strategy provides a scalable solution for tailoring Aspergillus niger enzymes to meet the growing demands of the prebiotic and functional food industries.
- 相关文献
作者其他论文 更多>>
-
Enhancing sugarcane growth, yield and quality parameters using varying application rates of potassium
作者：Bhatt, Rajan;Verma, Krishan K.;Singh, Jagdish;Kumar, Rajinder;Sharma, Anuradha;Mattar, Mohamed A.;Mattar, Mohamed A.
关键词：Sugarcane; potassium; insects; Pokkah boeng; CSS (t ha-1); benefits
-
Efficient micropropagation protocol and assessment of genetic fidelity of micropropagated plantlets of Pueraria tuberosa
作者：Kanthaliya, Bhanupriya;Joshi, Abhishek;Arora, Jaya;Verma, Krishan K.
关键词：Woody liana; genetic fidelity; biodiversity conservation; molecular marker;
in vitro propagation -
The regulatory mechanism controlling nitrification inhibitors-induced mitigation of nitrification and NO3--N leaching in alkaline purple soil
作者：Liu, Fabo;Zou, Wenxin;Lang, Ming;Li, Zhao Lei;Zhang, Fen;Wang, Xiaozhong;Chen, Xinping;Liu, Fabo;Lakshmanan, Prakash;Zou, Wenxin;Lang, Ming;Li, Zhao Lei;Zhang, Fen;Wang, Xiaozhong;Chen, Xinping;Lakshmanan, Prakash;Lakshmanan, Prakash;Lang, Ming;Li, Zhao Lei;Wang, Xiaozhong;Chen, Xinping;Lang, Ming;Li, Zhao Lei;Wang, Xiaozhong;Chen, Xinping;Liang, Tao;Chen, Jing;Wang, Yan
关键词：Nitrification inhibitors; Ammonia oxidizers; Vegetable; Purple soil
-
Application of Microbial Fermentation in Caffeine Degradation and Flavor Modulation of Coffee Beans
作者：Ran, Lu-Xia;Wei, Xiang-Ying;Ren, Er-Fang;Qin, Jian-Feng;Rasheed, Usman;Chen, Gan-Lin;Ran, Lu-Xia;Wei, Xiang-Ying;Rasheed, Usman;Chen, Gan-Lin;Ran, Lu-Xia;Wei, Xiang-Ying;Rasheed, Usman;Chen, Gan-Lin
关键词：decaffeination; low-caffeine coffee; microbial fermentation; mixed-culture; flavor enhancement
-
Response of alcohol fermentation strains, mixed fermentation and extremozymes interactions on wine flavor
作者：Zhao, Ling-Zhi;Zhao, Ling-Zhi;Chen, Jing;Wei, Xiang-Ying;Chen, Gan-Lin;Chen, Jing;Wei, Xiang-Ying;Chen, Gan-Lin;Chen, Jing;Wei, Xiang-Ying;Chen, Gan-Lin;Lin, Bo;Zheng, Feng-Jin;Verma, Krishan K.
关键词：alcohol fermentation; bacteria; extremophiles; single and mixed strain fermentation; strain improvement; wine flavor
-
Breeding and regional production capacity performance of new sugarcane cultivar GT 66
作者：Liang, Qiang;Liu, Xiao-yan;Zhou, Hui;Lei, Jing-chao;Lin, Shan-hai;Yan, Mei-xin;Verma, Krishan K.;Li, Yang-rui;Song, Xiu-peng;Li, Yi-jie;Wei, Kai-jun;Wei, Hong-chui;Li, Wen-jiao
关键词：Regional demonstration; Varietal breeding; Mechanization approach; Sugarcane cultivation; Guitang-66; Sustainable development
-
Assessment of acid production efficiency and aroma volatile compounds by immobilized fermentation of different carriers on sugarcane original vinegar
作者：Chen, Gan-Lin;Yang, Li-Fang;Chen, Gan-Lin;Chen, Gan-Lin;Lin, Bo;Zheng, Feng-Jin;Fang, Xiao-Chun;Lin, Bo;Zheng, Feng-Jin;Verma, Krishan K.
关键词：Corncobs; Rice husk; Sodium alginate; Volatile organic compounds; Sugarcane original vinegar
