Ultrastructural changes and the distribution of arabinogalactan proteins during somatic embryogenesis of banana (Musa spp. AAA cv. 'Yueyoukang 1')
文献类型： 外文期刊
作者： Pan, Xiao 1 ; Yang, Xiao 1 ; Lin, Guimei 2 ; Zou, Ru 1 ; Chen, Houbin 1 ; Samaj, Jozef 3 ; Xu, Chunxiang 1 ;
作者机构： 1.S China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
2.Guangxi Acad Agr Sci, Inst Biotechnol, Nanning 530007, Peoples R China
3.Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Dept Cell Biol, Fac Sci, Olomouc 78371, Czech Republic
关键词： monoclonal antibodies;starch: 9005-25-8;epitopes;arabinogalactan protein;protein distribution;cellular localization;subcellular localization;developmental regulation;somatic embryogenesis;ultrastructural change;developmental localization
期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：4.5； 五年影响因子：4.576 ）
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收录情况： SCI
摘要： A better understanding of somatic embryogenesis in banana (Musa spp.) may provide a practical way to improve regeneration of banana plants. In this study, we applied scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to visualize the ultrastructural changes during somatic embryogenesis of banana (Musa AAA cv. 'Yueyoukang 1'). We also used histological and immunohistochemical techniques with 16 monoclonal antibodies to study the spatial distribution and cellular/subcellular localization of different arabinogalactan protein (AGP) components of the cell wall during somatic embryogenesis. Histological study with periodic acid-Schiff staining documented diverse embryogenic stages from embryogenic cells (ECs) to the late embryos. SEM revealed a mesh-like structure on the surface of proembryos which represented an early structural marker of somatic embryogenesis. TEM showed that ECs were rich in juvenile mitochondria, endoplasmic reticulum and Golgi stacks. Cells in proembryos and early globular embryos resembled ECs, but they were more vacuolated, showed more regular nuclei and slightly more developed organelles. Immunocytochemical study revealed that the signal of most AGP epitopes was stronger in starch-rich cells when compared with typical ECs. The main AGP component in the extracellular matrix surface network of banana proembryos was the MAC204 epitope. Later, AGP immunolabelling patterns varied with the developmental stages of the embryos. These results about developmental regulation of AGP epitopes along with developmental changes in the ultrastructure of cells are providing new insights into the somatic embryogenesis of banana.
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