Two level half factorial design for the extraction of phenolics, flavonoids and antioxidants recovery from palm kernel by-product
文献类型： 外文期刊
作者： Wong, Wah Heng 1 ; Lee, Wei Xiong 1 ; Ramanan, Ramakrishnan Nagasundara 1 ; Tee, Lee Hong 1 ; Kong, Kin Weng 3 ; Ga 1 ;
作者机构： 1.Monash Univ, Sch Engn, Chem Engn Discipline, Bandar Sunway 47500, Selangor, Malaysia
2.Monash Univ, Sch Engn, Bandar Sunway 47500, Selangor, Malaysia
3.Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia
4.Galanakis Labs, Dept Res & Innovat, GR-73131 Khania, Greece
5.Guangxi Acad Agr Sci, Inst Agrofood Sci & Technol, Nanning 530007, Peoples R China
关键词： Antioxidants;Extraction;Flavonoids;Half factorial design;Palm kernel by-product
期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.645； 五年影响因子：5.749 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Every year palm oil industry in Malaysia produces enormous quantity of by-products and has great potential to be reutilized as good source of antioxidants. In this study, a two level half factorial design was employed to recover phenolics, flavonoids and antioxidants from palm kernel by-product. The assay included the following independent factors: pH (2-6), ethanol concentration (20-80%), liquid to solid ratio (10-100 mL/g), temperature (30-80 degrees C) and time (30-300 min). Half factorial design was employed to determine the significant contribution of the above factors towards antioxidant capacity (total antioxidant activity, DPPH and FRAP), total phenolic and flavonoid contents. Among, all the factors examined, ethanol concentration and liquid to solid ratio are very significant (p < 0.0001), in obtaining higher antioxidant activity, total phenolic and flavonoid contents. Thus, palm kernel by-product can be proposed to be used as natural antioxidant. (C) 2014 Elsevier B.V. All rights reserved.
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