您好，欢迎访问广西农业科学院 机构知识库！

The role of iron competition in the antagonistic action of Metschnikowia pulcherrima against three major pathogenic fungi on postharvest citrus fruit

文献类型： 外文期刊

作者： Wang, Shupei 1 ; Wang, Chenshu 3 ; Liu, Wenqing 3 ; Hang, Fangxue 3 ; Chen, Meilan 2 ; Zhao, Zhijuan 2 ; Ye, Dongqing 1 ; Li, Li 1 ; Zhang, Lan 1 ; He, Xuemei 1 ; Sun, Jian 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Guangxi Key Lab Fruits & Vegetables Storage Proc T, Nanning 530007, Peoples R China

2.Nanning Normal Univ, Coll Environm & Life Sci, Nanning 530001, Peoples R China

3.Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China

关键词： Metschnikowia pulcherrima; Citrus fruit; Pulcherriminic acid; Iron competition

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：5.3 ）

ISSN： 0168-1605

年卷期： 2025 年 441 卷

页码：

收录情况： SCI

摘要： This study systematically investigated how Metschnikowia pulcherrima XX04 competes with Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii for iron ions to control these major postharvest citrus pathogens. Exogenous Fe3+ addition significantly reduced the inhibitory activity of the pigment-producing yeast against these pathogens on both agar medium and fruit. Pearson correlation analysis revealed a strong positive relationship between pigment production and antagonistic effectiveness of M. pulcherrima XX04. Comparative analysis of iron-capturing proficiency between M. pulcherrima XX04 and three pathogenic fungi on PDA medium and citrus wounds indicated that M. pulcherrima XX04 exhibited superior iron sequestration capabilities. Colony growth viability assay performed on Pulcherrimin-containing PDA (Pul-PDA) solid medium showed M. pulcherrima XX04 could utilize pulcherrimin-bound iron for growth and metabolism, and further evidenced by the significant upregulation of PUL3, a putative transporter involved in the utilization of extracellular pulcherrimin-iron complexes, whereas the pathogens exhibited limited or no ability to assimilate iron from this source. Growth assay of three pathogens under iron-limited, iron-rich, and iron-replenish conditions showed that P. digitatum was the most sensitive to iron deficiency, followed by P. italicum, while G. citri-aurantii displayed the highest tolerance to low-iron environments. This observation was further validated by iron chelation sensitivity assays using the agar well diffusion method with two specialized chelators, 2,2 '-dipyridyl and tropolone. Overall, the pigment-producing yeast M. pulcherrima XX04 may have a greater capacity than the three tested pathogens to compete for and acquire iron in an iron-limited coexistence environment. This capability facilitates the induction of iron deprivation by M. pulcherrima against various fungal pathogens and represents a key determinant of its biocontrol efficacy. Our findings provide new insights into elucidating the mechanism of competitive iron consumption mediated by antagonistic yeasts. Furthermore, strategies based on inducing iron starvation could offer a promising alternative for managing postharvest diseases in citrus fruits.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台