Insight into the mechanism of efficient extraction of tea saponins from Camellia oleifera shells using deep eutectic solvents
文献类型： 外文期刊
作者： Mo, Xiangyan 1 ; Hang, Fangxue 1 ; Ren, Er-Fang 1 ; Gai, Lili 1 ; Li, Kai 1 ; Niu, Debao 1 ;
作者机构： 1.Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China
2.Guangxi Subtrop Crops Res Inst, Nanning 530001, Peoples R China
关键词： Camellia oleifera shells; Deep eutectic solvent; Tea saponins; UPLC-Q-Orbitrap HRMS; Molecular dynamic simulation; Extraction mechanism
期刊名称：INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES （ 影响因子：6.8； 五年影响因子：7.6 ）
ISSN： 1466-8564
年卷期： 2025 年 102 卷
页码：
收录情况： SCI
摘要： Camellia oleifera (C. oleifera) shells, a major by-product of the camellia oil industry, are rich in tea saponins. The efficient extraction and characterization of tea saponins from C. oleifera shells using deep eutectic solvents (DESs) were investigated, with the extraction mechanism further explored through scanning electron microscopy (SEM) and molecular dynamics simulations. A total of 24 different types of DESs were prepared, with the optimal DES identified as a combination of L-proline and acetamide (the molar ratio of 1:4). Response surface methodology (RSM) optimization revealed that the maximum yield of tea saponins extraction reached 22.46 %. 18 kinds of tea saponin monomers were identified by ultrahigh-performance liquid chromatography coupled with quadrupoleorbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). SEM analysis showed that DESs effectively disrupted the surface structure of C. oleifera shell. Furthermore, molecular dynamics simulations demonstrated the high extraction efficiency of DESs was due to their larger solvent accessible surface area (SASA), increased number of hydrogen bonds, extended hydrogen bond lifetime, and lower intermolecular interaction energy between DESs and tea saponins. These findings provide new insights into the extraction mechanism and contribute to the optimization of DES-based extraction techniques for bioactive compounds from natural plant sources.
- 相关文献
作者其他论文 更多>>
-
The role of iron competition in the antagonistic action of Metschnikowia pulcherrima against three major pathogenic fungi on postharvest citrus fruit
作者：Wang, Shupei;Ye, Dongqing;Li, Li;Zhang, Lan;He, Xuemei;Sun, Jian;Wang, Shupei;Chen, Meilan;Zhao, Zhijuan;Wang, Chenshu;Liu, Wenqing;Hang, Fangxue
关键词：Metschnikowia pulcherrima; Citrus fruit; Pulcherriminic acid; Iron competition
-
Revealing the multi-target destruction induced by proanthocyanidins against Acetobacter sp. at the molecular level
作者：Ren, Er-Fang;Feng, Xiaoqin;Feng, Yuanxin;Li, Kai;Chen, Shan;Niu, Debao;Ren, Er-Fang;Zeng, Xin-An;Cai, Jin-Lin;Han, Zhong;Wen, Qing-Hui
关键词：proanthocyanidins; cell membrane; membrane fatty acid; enzyme activity; genomic DNA; molecular docking
-
Application of Microbial Fermentation in Caffeine Degradation and Flavor Modulation of Coffee Beans
作者：Ran, Lu-Xia;Wei, Xiang-Ying;Ren, Er-Fang;Qin, Jian-Feng;Rasheed, Usman;Chen, Gan-Lin;Ran, Lu-Xia;Wei, Xiang-Ying;Rasheed, Usman;Chen, Gan-Lin;Ran, Lu-Xia;Wei, Xiang-Ying;Rasheed, Usman;Chen, Gan-Lin
关键词：decaffeination; low-caffeine coffee; microbial fermentation; mixed-culture; flavor enhancement
-
Rapid Identification of Soybean SC15 Resistance Locus to Soybean Mosaic Virus by BSA-seq
作者：Tang, Xiangmin;Chen, Huaizhu;Cai, Zhaoyan;Lai, Zhenguang;Wei, Qingyuan;Tan, Yurong;Chen, Dongliang;Zeng, Weiying;Yang, Shouzhen;Jin, Gang;Li, Kai;Zhi, Haijian
关键词：Soybean mosaic virus (SMV); Strain SC15; Bulked segregant analysis sequencing (BSA-seq); Euclidean distance (ED); SNP index
-
Investigation into potential allergenicity of DBD plasma-treated casein digestion products based on immunoglobulin E linear epitopes and the sensitized-cell model
作者：Zhang, Yongniu;Gai, Lili;Chen, Siyu;Li, Kai;Niu, Debao;Ren, Er -Fang;Wen, Tao;Lyu, Shijun;Han, Zhong;Niu, Fuge
关键词：Casein; DBD plasma treatment; Allergenicity; In vitro digestion; Structure; Bioinformatics analysis
-
Establishment of efficient system for bagasse bargaining: Combining fractionation of saccharides, recycling of high-viscosity solvent and dismantling
作者：Li, Yihan;Kang, Xiheng;You, Zi;Song, Xueping;Li, Kai;He, Tieguang;Su, Tianming;Zhang, Junhua;Li, Yihan;Li, Kai;Zhuang, Xinshu;Zhang, Zhanying;Ragauskas, Arthur J.
关键词：Sugarcane bagasse; Dismantling; High-viscosity system; Saccharides; Solvent reusing; Solvent reusing
-
Iron Competition as an Important Mechanism of Pulcherrimin-Producing Metschnikowia sp. Strains for Controlling Postharvest Fungal Decays on Citrus Fruit
作者：Wang, Shupei;He, Xuemei;Ye, Dongqing;Li, Li;Sun, Jian;Wang, Shupei;He, Xuemei;Ye, Dongqing;Li, Li;Sun, Jian;Wang, Shupei;Tan, Zhimei;Wang, Chenshu;Liu, Wenqing;Hang, Fangxue
关键词：Metschnikowia pulcherrima; pulcherrimin; iron competition; P. digitatum; P. italicum; G. citri-aurantii
