Investigation into potential allergenicity of DBD plasma-treated casein digestion products based on immunoglobulin E linear epitopes and the sensitized-cell model
文献类型： 外文期刊
作者： Zhang, Yongniu 1 ; Ren, Er -Fang 2 ; Wen, Tao 3 ; Lyu, Shijun 3 ; Gai, Lili 1 ; Chen, Siyu 1 ; Li, Kai 1 ; Han, Zhong 4 ; Niu, Fuge 5 ; Niu, Debao 1 ;
作者机构： 1.Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China
2.Guangxi Subtrop Crops Res Inst, Nanning 530001, Peoples R China
3.Guangxi Zhuang Autonomous Reg Testing Inst Prod Qu, Nanning 530200, Peoples R China
4.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510641, Peoples R China
5.Zhejiang Gongshang Univ, Sch Food Sci & Biotechnol, Food Safety Key Lab Zhejiang Prov, Hangzhou 310018, Peoples R China
关键词： Casein; DBD plasma treatment; Allergenicity; In vitro digestion; Structure; Bioinformatics analysis
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2024 年 447 卷
页码：
收录情况： SCI
摘要： The study aimed to investigate the allergenicity change in casein treated with dielectric barrier discharge (DBD) plasma during in vitro simulated digestion, focusing on the immunoglobulin E (IgE) linear epitopes and utilizing a sensitized -cell model. Results indicated that prior treatment with DBD plasma treatment (4 min) before simulated digestion led to a 10.5% reduction in the IgE-binding capacity of casein digestion products. Moreover, the release of biologically active substances induced from KU812 cells, including beta-HEX release rate, human histamine, IL -4, IL -6, and TNF-alpha, decreased by 2.1, 28.1, 20.6, 11.6, and 17.3%, respectively. Through a combined analysis of LCMS/MS and immunoinformatics tools, it was revealed that DBD plasma treatment promoted the degradation of the IgE linear epitopes of casein during digestion, particularly those located in the alpha-helix region of alpha s1-CN and alpha s2-CN. These findings suggest that DBD plasma treatment prior to digestion may alleviate casein allergic reactions.
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