文献类型： 外文期刊

作者： Gao, Meiping ¹ ; Jiang, Wen ¹ ; Wei, Shaolong ¹ ; Lin, Zhicheng ² ; Cai, Binghua ¹ ; Yang, Liu ¹ ; Luo, Cong ¹ ; He, Xinh ¹ ;

作者机构： 1.Guangxi Acad Agr Sci, Biotechnol Res Inst, Nanning 530007, Guangxi, Peoples R China

2.Guangxi Tissue Cultured Seedlings Co Ltd, Nanning 530007, Guangxi, Peoples R China

3.Guangxi Univ, Coll Agr, Nanning 530007, Guangxi, Peoples R China

关键词： Chinese water chestnut;Temporary immersion bioreactor system (TIBS);Semi-solid medium;In vitro cell culture;Root formation

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

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收录情况： SCI

摘要： Several studies have reported different propagation methods for the Chinese water chestnut [Eleocharis dulcis (Burm.f.) Trin. ex Hensch]. To date, none of these methods have efficiently achieved large-scale plantlet production using minimal labor input. The present study investigated the propagation of Chinese water chestnut shoot tips derived from corm and creeping rhizomes in a temporary immersion bioreactor system (TIBS) compared with propagation using conventional semi-solid medium methods. We evaluated the regeneration capacity of the corm and creeping rhizome shoot tips of E. dulcis Guiti No. 2 sp. nov. and found that culturing under optimized TIBS conditions (15 min of immersion into nutrient medium every 8 h) produced the highest multiplication rate (36.5 times) and shoot-forming capacity (SFC, 68.9), and reduced the vitrification rate. Creeping rhizome shoot tips had slightly higher SFC than corm shoot tips; the highest shoot multiplication rate of 43.7 times in TIBS was obtained using Murashige-Skoog (MS) medium containing 4 mg L-1 6-benzylaminopurine and 0.5 mg L-1 1-naphthaleneacetic acid (NAA). These optimized conditions were applied to corm shoot tips of E. dulcis Guiti No. 1 var. nov., E. dulcis Guiti No. 2 sp. nov., and E. dulcis Guifengti No. 1 sp. nov. Root formation was observed during the shoot multiplication stage on semi-solid MS medium; however, root formation was not noted during the shoot multiplication stage in TIBS. While TIBS produced a higher multiplication rate and SFC indices, there were no significant differences in average number of shoots per explant between TIBS and semi-solid medium. We also evaluated the effects of different immersion frequencies and NAA concentrations on TIBS propagation of E. dulcis Guiti No. 2 sp. nov. shoot tips by measuring the rooting percentage, average number of roots (length a parts per thousand yen 3 cm) per explant, maximum root length, and root-forming capacity (RFC). The results showed that the medium containing 2.0 mg L-1 NAA at the rooting stage produced the best-developed root systems with the highest rooting percentage, maximum root length, and highest RFC (75.8 %, 8.5 cm, and 3.8, respectively); it also reduced the immersion frequency. The optimized conditions produced plantlets that had a greater capacity to acclimatize after transfer to the greenhouse. E. dulcis Guiti No. 1 var. nov. plantlets had the highest survival rate (95 %), largest diameters, and highest number of creeping rhizomes. The results of this study demonstrate that TIBS is a reliable and efficient method for large-scale propagation of Chinese water chestnut plantlets.