文献类型： 外文期刊

作者： Wu, Yu ¹ ; Chen, Haonan ¹ ; Wu, Mengbo ¹ ; Zhou, Yuanyi ¹ ; Yu, Chuying ² ; Yang, Qihong ² ; Rolland, Filip ³ ; van de Poel, Bram ⁴ ; Bouzayen, Mondher ⁶ ; Hu, Nan ⁷ ; Wang, Yikui ² ; Liu, Mingchun ¹ ;

作者机构： 1.Sichuan Univ, Coll Life Sci, Key Lab Bioresource & Ecoenvironm, Minist Educ, Chengdu 610065, Sichuan, Peoples R China

2.Guangxi Acad Agr Sci, Vegetable Res Inst, Nanning 530007, Guangxi, Peoples R China

3.Katholieke Univ Leuven, Dept Biol, Lab Plant Metab Signaling, Kasteelpk Arenberg 31, B-3001 Heverlee, Belgium

4.Katholieke Univ Leuven, Plant Inst, Kasteelpk Arenberg 31, B-3001 Heverlee, Belgium

5.Katholieke Univ Leuven, Dept Biosyst, Div Crop Biotech, Mol Plant Hormone Physiol Lab, Willem Croylaan 42, B-3001 Heverlee, Belgium

6.Univ Toulouse, Lab Rech Sci Vegetales Genom & Biotechnol Fruits, CNRS, UMR5546,UPS, F-31320 Toulouse, France

7.Anyang Inst Technol, Coll Biol & Food Engn, Anyang 455000, Henan, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）

ISSN： 2662-6810

年卷期： 2025 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Sugars act as signaling molecules to modulate various growth processes and enhance plant tolerance to various abiotic and biotic stresses. Moreover, sugars contribute to the postharvest flavor in fleshy fruit crops. To date, the regulation of sugar metabolism and its effect in plant growth, fruit ripening, postharvest quality, and stress resistance remains not fully understood. In this study, we investigated the role of tomato gene encoding a vacuolar invertase, hydrolyzing sucrose to glucose and fructose. SlVI is specifically expressed during the tomato fruit ripening process. We found that overexpression of SlVI resulted in increased leaf size and early flowering, while knockout of SlVI led to increased fruit sucrose content, enhanced fruit firmness, and elevated resistance of postharvest fruit to Botrytis cinerea. Moreover, the content of naringenin and total soluble solids was significantly increased in SlVI knockout fruit at postharvest stage. Transcriptome analysis showed a negative feedback regulation triggered by sucrose accumulation in SlVI knockout fruit resulting in a downregulation of BAM3 and AMY2, which are critical for starch degradation. Moreover, genes associated with cell wall, cutin, wax, and flavonoid biosynthesis and pathogen resistance were upregulated in SlVI knockout fruit. Conversely, the expression levels of genes involved in cell wall degradation were decreased in knockout fruit. These results are consistent with the enhanced postharvest quality and resistance. Our findings not only provide new insights into the relationship between tomato fruit sucrose content and postharvest fruit quality, but also suggest new strategies to enhance fruit quality and extend postharvest shelf life.