Olfactory co-receptor is involved in host recognition and oviposition in Ophraella communa (Coleoptera: Chrysomelidae)
文献类型： 外文期刊
作者： Ma, Chao 1 ; Cui, Shaowei 1 ; Bai, Qiang 1 ; Tian, Zhenya 1 ; Zhang, Yan 1 ; Chen, Guangmei 1 ; Gao, Xuyuan 1 ; Tian, Zhe 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Jilin Agr Univ, Sch Plant Protect, Changchun, Peoples R China
3.Yunnan Agr Univ, Coll Plant Protect, Kunming, Yunnan, Peoples R China
4.Guangxi Acad Agr Sci, Inst PlantProtect, Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning, Peoples R China
关键词： Ophraella communa; odorant receptor; co-receptor; RNA interference; electroantennogram; behavioural test; reproduction
期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）
ISSN： 0962-1075
年卷期： 2020 年 29 卷 4 期
页码：
收录情况： SCI
摘要： Common ragweed (Ambrosia artemisiifolia) is a notorious invasive weed that has spread across most temperate regions of the world. The beetle (Ophraella communa) is considered to be an effective control agent against A. artemisiifolia. As an oligophagous insect, its olfactory system is extremely important for host seeking in the wild. To the best of our knowledge, there is no report on the molecular mechanisms underlying olfaction recognition in this beetle. Hence, in this study, we characterized the odorant receptor co-receptor of O. communa and named it as 'OcomORco'. Real-time quantitative PCR (qRT-PCR) showed that, compared to the control treatment, RNA interference (RNAi) strongly reduced the expression of OcomORco by 89% in male and 90% in female beetles. Electroantennogram assay showed that the antennal response of both male and female beetles to four volatiles of A. artemisiifolia was significantly reduced. The injected male or female beetles lost their preference for plant leaves as observed in the behavioural tests. In addition, disruption of the expression of OcomORco resulted in a reduction of oviposition, while there was no difference in larval hatching rate between control and knockdown females. We demonstrated that OcomORco plays a vital role in olfactory perception and host search in O. communa, and it is involved in oviposition in an indirect way.
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