Fluorescence immunoassay for multiplex detection of organophosphate pesticides in agro-products based on signal amplification of gold nanoparticles and oligonucleotides
文献类型： 外文期刊
作者： Zhang, Chan 1 ; Jiang, Zejun 2 ; Jin, Maojun 1 ; Du, Pengfei 3 ; Chen, Ge 1 ; Cui, Xueyan 1 ; Zhang, Yudan 1 ; Qin, Guoxi 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Qual & Safety, Key Lab Agroprod Qual & Safety,Minist Agr, Beijing 100081, Peoples R China
2.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China
3.Shandong Acad Agr Sci, Key Lab Agroprod Proc Technol Shandong Prov, Key Lab Novel Food Resources Proc, Inst Agrofood Sci & Technol,Minist Agr, Jinan 250100, Peoples R China
4.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning 530007, Peoples R China
5.Qilu Univ Technol, Shandong Acad Sci, Coll Food Sci & Engn, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Peoples R China
6.Cairo Univ, Dept Pharmacol, Fac Vet Med, Giza 12211, Egypt
7.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkey
关键词： Fluorescence immunoassay; Organophosphate pesticides; Oligonucleotide; Gold nanoparticle
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2020 年 326 卷
页码：
收录情况： SCI
摘要： Herein, we developed a multi-analyte fluorescence immunoassay for detection of three organophosphate pesticides (triazophos, parathion, and chlorpyrifos) in various agro-products (rice, wheat, cucumber, cabbage, and apple) using fluorescently labeled oligonucleotide and gold nanoparticle (AuNP) signal amplification technology. The AuNP probes for the three analytes were constructed by simultaneously modifying the corresponding antibodies and fluorescently labeled oligonucleotides on the probe surface. Three fluorophores (6-FAM, Cy3, and Texas red) with high fluorescence intensity and little overlap of excitation/emission wavelengths were selected. The method showed satisfactory linearity for triazophos, parathion, and chlorpyrifos in the ranges of 0.01-20, 0.05-50, and 0.5-1000 mu g/L, respectively. For the 3 analytes, the limits of detection (LODs) were 0.007, 0.009, and 0.087 mu g/L, respectively. The average recoveries were 77.7-113.6%, with relative standard deviations (RSDs) of 7.1-17.1% in various food matrices. The proposed method offers great potential in food safety surveillance, and could be used as well as a reference for multi-residue analysis of other small-molecule contaminants.
- 相关文献
作者其他论文 更多>>
-
An intelligent sensing platform for sugarcane pokkah boeng disease based on CHA-driven RCA cascade DNAzyme amplification technology and clustered DNA nanoflowers
作者：Wu, Qingnian;Song, Yujie;Jin, Chenchen;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu;Wang, Zeping;Liao, Jie;Yan, Feiyan
关键词：Sugarcane pokkah boeng disease; Cascade amplification technology; Bundle DNA nanoflowers; Smartphone assisted detection
-
Sensitivity-enhanced self-powered biosensing platform for detection of sugarcane smut using Mn-doped ZIF-67, RCA-DNA nano-grid array and capacitor
作者：Fu, Bingtao;Che, Rongshuai;Wu, Yeyu;Luo, Hu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Ya, Yu
关键词：Sugarcane smut; Self-powered platform; DNA nano-grid array; Cascade signal amplification
-
From leaf to lab-on-cloth: Spatial DNA nanorobotics and 2D graphyne synergy enable ultra-precise electrochemical tracking of sugarcane pokkah boeng disease
作者：Wu, Qingnian;Jin, Chenchen;Zhao, Yinxia;Feng, Defen;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu;Yan, Feiyan;Xie, Shengyu
关键词：Sugarcane pokkah boeng disease; S-GDY; Dual strand displacement amplification; Dual-signal readout; Spatial DNA nanorobotics
-
High-Performance Biosensing Platforms Based on Enzyme-Linked Nucleic Acid Amplification Regulated by Synergistic Allosteric Hairpin Catalysis of Bimetallic Nanozymes and Its Mechanisms
作者：Ma, Yunzhi;Jin, Chenchen;Wu, Yeyu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Ya, Yu
关键词：
-
Passion fruit peel pectin improves glucose and lipid metabolism in high-fat diet-induced obesity through gut microbiota and insulin sensitivity regulation
作者：Ju, Ying;Luo, Hailing;Huang, Yongcai;Chen, Ge;Gui, Jie;Lin, Shengli;Yang, Liu;Ju, Ying;Huang, Yongcai;Chen, Ge;Gui, Jie;Yang, Liu;Huang, Liling;Liu, Xuzhou;Huang, Xianya
关键词：Passion fruit peel pectin; Insulin resistance; PI3K-AKT pathway
-
Comparative Metabolomics Analysis of Four Pineapple (Ananas comosus L. Merr) Varieties with Different Fruit Quality
作者：Zheng, Ping;Wu, Jiahao;Li, Denglin;Xie, Shiyu;Cai, Xinkai;Xiao, Qiang;Wang, Jing;Yao, Qinglong;Chen, Shengzhen;Qin, Yuan;Wang, Xiaomei;Xiao, Qiang;Deng, Biao;Wang, Xiaomei;Liu, Ruoyu;Qin, Yuan;Liang, Yuqin;Zhang, Yangmei
关键词：pineapple; metabolome; fruit quality; comparative analysis
-
Dual-modal improved biosensing platform for sugarcane smut pathogen based on biological enzyme-Mg2+ DNAzyme coupled with DNA transporter cascading hybridization chain reaction
作者：Che, Rongshuai;Tang, Danyao;Fu, Bingtao;Wu, Yeyu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Yan, Meixin;Ya, Yu
关键词：3D DNA transporter; Sugarcane smut pathogen; Dual-modal detection
