The Impact of AAPH-Induced Oxidation on the Functional and Structural Properties, and Proteomics of Arachin
文献类型： 外文期刊
作者： Shen, Mingjuan 1 ; Yang, Xi 1 ; Wang, Zhenxing 1 ; Sha, Xiaomei 2 ; Zhang, Xuechun 1 ; Sun, Jian 3 ;
作者机构： 1.Southwest Forestry Univ, Coll Life Sci,Minist Educ, Key Lab Forest Resources Conservat & Utilizat Sout, Key Lab Forest Disaster Warning & Control Yunnan P, Kunming 650224, Peoples R China
2.Jiangxi Normal Univ, Coll Life Sci, Natl R&D Ctr Freshwater Fish Proc, Nanchang 330022, Peoples R China
3.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China
关键词： arachin; AAPH; oxidation; functional properties; structural characteristics; proteomics
期刊名称：MOLECULES （ 影响因子：4.6； 五年影响因子：4.9 ）
ISSN：
年卷期： 2023 年 28 卷 17 期
页码：
收录情况： SCI
摘要： The aim of this study was to investigate the effect of 2,2 & PRIME;-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidation on the functional, structural properties and proteomic information of arachin. The results showed that moderate oxidation improved the water/oil holding capacity of proteins and increased the emulsifying stability, while excessive oxidation increased the carbonyl content, reduced the thiol content, altered the structure and thermal stability, and reduced most of the physicochemical properties. Through LC-QE-MS analysis, it was observed that oxidation leads to various modifications in arachin, including carbamylation, oxidation, and reduction, among others. In addition, 15 differentially expressed proteins were identified. Through gene ontology (GO) analysis, these proteins primarily affected the cellular and metabolic processes in the biological process category. Further Kyoto encyclopedia of genes and genomes (KEGG) analysis revealed that the "proteasome; protein processing in the endoplasmic reticulum (PPER)" pathway was the most significantly enriched signaling pathway during the oxidation process of arachin. In conclusion, this study demonstrated that AAPH-induced oxidation can alter the conformation and proteome of arachin, thereby affecting its corresponding functional properties. The findings of this study can potentially serve as a theoretical basis and foundational reference for the management of peanut processing and storage.
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