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Sugarcane Root Transcriptome Analysis Revealed the Role of Plant Hormones in the Colonization of an Endophytic Diazotroph

文献类型： 外文期刊

作者： Nong, Qian 1 ; Malviya, Mukesh Kumar 1 ; Solanki, Manoj Kumar 3 ; Solanki, Anjali Chandrol 4 ; Lin, Li 1 ; Xie, Jinlan 1 ; Mo, Zhanghong 1 ; Wang, Zeping 1 ; Song, Xiu-Peng 1 ; Huang, Xin 1 ; Rai, Shalini 5 ; Li, Changning 1 ; Li, Yang-Rui 1 ;

作者机构： 1.Chinese Acad Agr Sci, Sugarcane Res Inst, Guangxi Acad Agr Sci, Key Lab Sugarcane Biotechnol & Genet Improvement G, Nanning, Peoples R China

2.Guangxi Acad Agr Sci, Plant Protect Res Inst, Nanning, Peoples R China

3.Univ Siles Katowice, Inst Biol Biotechnol & Environm Protect, Fac Nat Sci, Plant Cytogenet & Mol Biol Grp, Katowice, Poland

4.Mansarovar Global Univ, Dept Agr Sci, Bhopal, India

5.Soc Higher Educ & Pract Applicat SHEPA, Dept Biotechnol, Varanasi, India

关键词： sugarcane root; physiological function; gene expression; endophytic diazotroph; inoculation

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Some sugarcane germplasms can absorb higher amounts of nitrogen via atmospheric nitrogen fixation through the bacterial diazotrophs. Most endophytic diazotrophs usually penetrate through the root, colonize inside the plant, and fix the nitrogen. To assess the plant's bacterial association during root colonization, strain GXS16 was tagged with a plasmid-bear green fluorescent protein (GFP) gene. The results demonstrated that the strain can colonize roots all the way to the maturation zone. The strain GXS16 showed maximum nitrogenase enzyme activity at pH 8 and 30 degrees C, and nitrogenase activity is less affected by different carbon sources. Further, strain GXS16 colonization response was investigated through plant hormones analysis and RNAseq. The results showed that the bacterial colonization gradually increased with time, and the H2O2 and malondialdehyde (MDA) content significantly increased at 1 day after inoculation. There were no substantial changes noticed in proline content, and the ethylene content was detected initially, but it decreased with time. The abscisic acid (ABA) content showed significant increases of 91.9, 43.9, and 18.7%, but conversely, the gibberellin (GA(3)) content decreased by 12.9, 28.5, and 45.2% at 1, 3, and 5 days after inoculation, respectively. The GXS16 inoculation significantly increased the activities of catalase (CAT), superoxide dismutase (SOD), polyphenol oxidase (PPO), ascorbate peroxidase (APX), and glutathione reductase (GR) at different timepoint. In contrast, the peroxisome (POD) activity had no changes detected during the treatment. In the case of RNAseq analysis, 2437, 6678, and 4568 differentially expressed genes (DEGs) were identified from 1, 3, and 5 days inoculated root samples, and 601 DEGs were shared in all samples. The number or the expression diversity of DEGs related to ethylene was much higher than that of ABA or GA, which indicated the critical role of ethylene in regulating the sugarcane roots response to GXS16 inoculation.

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[1]Sugarcane Root Transcriptome Analysis Revealed the Role of Plant Hormones in the Colonization of an Endophytic Diazotroph. Nong, Qian,Malviya, Mukesh Kumar,Lin, Li,Xie, Jinlan,Mo, Zhanghong,Wang, Zeping,Song, Xiu-Peng,Huang, Xin,Li, Changning,Li, Yang-Rui,Nong, Qian,Solanki, Manoj Kumar,Solanki, Anjali Chandrol,Rai, Shalini. 2022

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