BSA-seq and genetic mapping reveals AhRt2 as a candidate gene responsible for red testa of peanut
文献类型： 外文期刊
作者： Zhang, Kun 1 ; Yuan, Mei 3 ; Xia, Han 1 ; He, Liangqiong 5 ; Ma, Jing 1 ; Wang, Mingxiao 1 ; Zhao, Huiling 1 ; Hou, Lei 1 ; Zhao, Shuzhen 1 ; Li, Pengcheng 1 ; Tian, Ruizheng 1 ; Pan, Jiaowen 1 ; Li, Guanghui 1 ; Thudi, Mahendar 1 ; Ma, Changle 2 ; Wang, Xingjun 1 ; Zhao, Chuanzhi 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Inst Biotechnol, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China
3.Shandong Peanut Res Inst, Qingdao 266199, Shandong, Peoples R China
4.Shandong Agr & Engn Univ, Coll Agr Sci & Technol, Jinan 250100, Peoples R China
5.Guangxi Acad Agr Sci, Cash Crop Res Inst, Nanning 530007, Peoples R China
6.Hainan Univ, Coll Trop Crops, Haikou 572208, Hainan, Peoples R China
7.Dr Rajendra Prasad Cent Agr Univ, Samsthipur 848125, Bihar, India
期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）
ISSN： 0040-5752
年卷期：
页码：
收录情况： SCI
摘要： Key message The candidate recessive gene AhRt2 responsible for red testa of peanut was identified through combined BSA-seq and linkage mapping approaches. The testa color of peanuts (Arachis hypogaea L.) is an important trait, and those with red testa are particularly popular owing to the high-anthocyanin content. However, the identification of genes underlying the regulation of the red testa trait in peanut are rarely reported. In order to fine map red testa gene, two F-2:4 populations were constructed through the cross of YZ9102 (pink testa) with ZH12 (red testa) and ZH2 (red testa). Genetic analysis indicated that red testa was controlled by a single recessive gene named as AhRt2 (Red testa gene 2). Using BSA-seq approach, AhRt2 was preliminary identified on chromosome 12, which was further mapped to a 530-kb interval using 220 recombinant lines through linkage mapping. Furthermore, functional annotation, expression profiling, and the analyses of sequence variation confirmed that the anthocyanin reductase namely (Arahy.IK60LM) was the most likely candidate gene for AhRt2. It was found that a SNP in the third exon of AhRt2 altered the encoding amino acids, and was associated with red testa in peanut. In addition, a closely linked molecular marker linked with red testa trait in peanut was also developed for future studies. Our results provide valuable insight into the molecular mechanism underlying peanut testa color and present significant diagnostic marker resources for marker-assisted selected breeding in peanut.
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