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Production of polyunsaturated fatty acids by Mucor recurvus sp with sugarcane molasses as the carbon source

文献类型： 外文期刊

作者： Li, Nan 1 ; Deng, Zhi-Nian 2 ; Qin, Yong-Ling 1 ; Chen, Chun-Lan 1 ; Liang, Zhi-Qun 1 ;

作者机构： 1.Guangxi Univ, Coll Life Sci & Technol, CN-530004 Nanning, Guangxi, Peoples R China

2.Guangxi Acad Agr Sci, Key Lab Crop Genet Improvement & Biotechnol, CN-530007 Nanning, Guangxi, Peoples R China

关键词： Mucor recurvus sp.;polyunsaturated fatty acids;sugarcane molasses;fermentation;cultivation condition;fatty acid analysis

期刊名称：FOOD TECHNOLOGY AND BIOTECHNOLOGY （ 影响因子：3.918； 五年影响因子：3.71 ）

ISSN： 1330-9862

年卷期： 2008 年 46 卷 1 期

页码：

收录情况： SCI

摘要： Sugarcane molasses is rich in nutriments and vitamins. It may be used as the carbon source for the production of polyunsaturated fatty acids (PUFA) by Mucor recurvus sp. Using sugarcane molasses, the effects of fermentation parameters and media components on polyunsaturated fatty acid production through both linear and orthogonal array experiments were investigated. The best fermentation conditions for PUFA production were found as follows: 15 % sugarcane molasses, pH=6.0, 28 degrees C, 5 days, 160 rpm. It was also found that molasses and urea enhanced PUFA production with the optimal carbon to nitrogen (C/N) ratio of 35. Under the most favourable conditions, the total lipid content at 7.13 g/L and PUFA up to 5.74 g/L including (0.82 +/- 0.05) g/L of linolenic acid (LA), (1.35 +/- 0.02) g/L Of gamma-linolenic acid (GLA), (0.17 +/- 0.06) g/L of a-linolenic acid (ALA), (0.57 +/- 0.06) g/L of arachidonic acid (ARA), (0.46 +/- 0.07) g/L of eicosapentaenoic acid (EPA) and (0.34 +/- 0.08) g/L of docosahexaenoic acid (DHA) were obtained. Our study suggests that sugarcane molasses is a superior alternative carbon source for industrial PUFA production.

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